Abstract
A new, sensitive and simple method for the rapid quantitative determination of ibuprofen in human plasma has been developed. This method involves the use of a solid phase extraction on “Baker” C-18 disposable extraction columns for sample clean-up and uses mefenamic acid as an internal standard.
Separation and quantitation are performed by reversed-phase liquid chromatography using a Nucleosil C18 column and methanol−0.04 M phosphoric acid (80:20, v/v), as the mobile phase.
Detection was achieved by UV-absorbance measurements at 229 nm.
Separation and quantitation are performed by reversed-phase liquid chromatography using a Nucleosil C18 column and methanol−0.04 M phosphoric acid (80:20, v/v), as the mobile phase.
Detection was achieved by UV-absorbance measurements at 229 nm.
| Original language | English |
|---|---|
| Pages (from-to) | 433-438 |
| Number of pages | 6 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1985 |