Determination of ibuprofen in human plasma by solid phase extraction and reversed-phase high-performance liquid chromatography

J. H. G. Jonkman*, R. Schoenmaker, A. H. Holtkamp, J. Hempenius

*Corresponding author for this work

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    A new, sensitive and simple method for the rapid quantitative determination of ibuprofen in human plasma has been developed. This method involves the use of a solid phase extraction on “Baker” C-18 disposable extraction columns for sample clean-up and uses mefenamic acid as an internal standard.

    Separation and quantitation are performed by reversed-phase liquid chromatography using a Nucleosil C18 column and methanol−0.04 M phosphoric acid (80:20, v/v), as the mobile phase.

    Detection was achieved by UV-absorbance measurements at 229 nm.
    Original languageEnglish
    Pages (from-to)433-438
    Number of pages6
    JournalJournal of Pharmaceutical and Biomedical Analysis
    Issue number5
    Publication statusPublished - 1985

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