Develoment of a reliable remote-controlled synthesis of beta-[C-11]-5-hydroxy-L-tryptophan on a Zymark robotic system

O. C. Neels*, P. L. Jager, K. P. Koopmans, E. Eriks, E. G. E. de Vries, I. P. Kema, P. H. Elsinga

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Precise staging of neuroendocrine tumors (NET) using positron emission tomography (PET) tracers visualizing their specific metabolic activity is of interest. Besides [F-18]FDOPA, staging NET with carbon-11 labeled 5-hydroxytryptophan (5-HTP) is reported in recent literature. We implemented the multi-enzymatic synthesis of enantiomerically pure [C-11]-L-5-HTP on a Zymark robotic system to compare both tracers in patient studies. [C-11]-5-HTP can be synthesized in up to 24% radiochemical yields (EOB). Average specific activity is 44000 GBq/mmol in ca. 50 min from [C-11]methyl iodide in radiochemical purities > 99 %. The synthesis of 5-HTP is difficult due to its multi-enzymatic reaction steps but typical yields can be achieved of ca. 400 MBq. [C-11]-5-HTP is now reliably used in ongoing studies for staging NET. Copyright (c) 2006 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)889-895
Number of pages7
JournalJOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
Volume49
Issue number10
DOIs
Publication statusPublished - Sep-2006

Keywords

  • POSITRON-EMISSION-TOMOGRAPHY
  • CARCINOID-TUMORS
  • L-TRYPTOPHAN
  • 5-HYDROXY-L-TRYPTOPHAN
  • ACIDS

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