Development and RNA-synthesizing activity of coronavirus replication structures in the absence of protein synthesis

Sjoerd H E van den Worm, Kèvin Knoops, Jessika C Zevenhoven-Dobbe, Corrine Beugeling, Yvonne van der Meer, A Mieke Mommaas, Eric J Snijder

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The RNA replication and transcription complex of coronaviruses is associated with an elaborate reticulovesicular network (RVN) of modified endoplasmic reticulum. Using cycloheximide and puromycin, we have studied the effect of translation inhibition on the RNA synthesis of severe acute respiratory syndrome coronavirus and mouse hepatitis virus. Both inhibitors prevented the usual exponential increase in viral RNA synthesis, with immunofluorescence and electron microscopy indicating that RVN development came to a standstill. Nevertheless, limited RNA synthesis was supported, implying that continued translation is not an absolute requirement and suggesting a direct link between RVN formation and accumulation of coronavirus proteins.

Original languageEnglish
Pages (from-to)5669-73
Number of pages5
JournalJournal of Virology
Volume85
Issue number11
DOIs
Publication statusPublished - Jun-2011
Externally publishedYes

Keywords

  • Animals
  • Cercopithecus aethiops
  • Cycloheximide
  • Microscopy, Electron, Transmission
  • Microscopy, Fluorescence
  • Murine hepatitis virus
  • Protein Biosynthesis
  • Protein Synthesis Inhibitors
  • Puromycin
  • RNA, Viral
  • SARS Virus
  • Transcription, Genetic
  • Vero Cells
  • Viral Proteins
  • Virus Replication

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