Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups

HJM Harmsen, ACM Wildeboer-Veloo, J Grijpstra, J Knol, JE Degener, GW Welling*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

252 Citations (Scopus)

Abstract

Two 16S rRNA-targeted probes were developed: one for the Coriobacterium group and the other for the Atopobium cluster (which comprises most of the Coriobacteriaceae species, including the Coriobacterium group). The new probes were based on sequences of three new Coriobacteriaceae strains isolated from human feces and clinical material and sequences from databases. Application of the probes to fecal samples showed that formula-fed infants had higher numbers of Coriobacterium group cells in their feces than breast-fed infants. In addition, based on the presented results, it is hypothesized that with the increasing age of a person, the diversity of Atopobium cluster species present in the feces increases.

Original languageEnglish
Pages (from-to)4523-4527
Number of pages5
JournalApplied and environmental microbiology
Volume66
Issue number10
Publication statusPublished - Oct-2000

Keywords

  • TARGETED OLIGONUCLEOTIDE PROBES
  • IN-SITU HYBRIDIZATION
  • HUMAN FECAL SAMPLES
  • RIBOSOMAL-RNA
  • COMB. NOV
  • PHENOTYPIC EVIDENCE
  • EUBACTERIUM-LENTUM
  • SEQUENCE-ANALYSIS
  • GEN. NOV.
  • COMMUNITIES

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