Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

Louis van de Zande, Steven Ferber, Ammerins de Haan, Leo W. Beukeboom, Joost van Heerwaarden, Bart A. Pannebakker*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (N-e) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.

Original languageEnglish
Pages (from-to)578-587
Number of pages10
JournalMolecular Ecology Resources
Volume14
Issue number3
DOIs
Publication statusPublished - May-2014

Keywords

  • parasitoid wasp
  • pooled resequencing
  • effective population size
  • single-nucleotide polymorphism (SNP)
  • genetic variation
  • laboratory strain
  • PARASITOID WASP NASONIA
  • LOCAL MATE COMPETITION
  • SEX-RATIO
  • DROSOPHILA-MELANOGASTER
  • NATURAL-POPULATIONS
  • ECTOPARASITIC WASP
  • HYMENOPTERA
  • GENOME
  • EVOLUTION
  • RECOMBINATION

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