Development of a Novel Orthogonal Double Gradient for High-Throughput Screening of Mesenchymal Stem Cells-Materials Interaction

Qihui Zhou, Lu Ge, Carlos F. Guimaraes, Philipp T. Kuhn, Liangliang Yang, Patrick van Rijn*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Surface gradients provide a powerful platform to accelerate multiscale design by efficiently studying of material-cell interactions to ultimately enhance function of synthetic clinical biomaterials. Herein, a novel orthogonal double gradient is reported in which surface stiffness and wettability vary independently and continuously in perpendicular directions, providing unique combinations of stiffness and wettability over a broad range (stiffness: 6-89 MPa; water contact angle: 29 degrees-90 degrees). It is found that mesenchymal stem cell behavior is nonlinearly regulated by surface stiffness and wettability. These combined stiffness and wettability properties of a material significantly affect stem cell adhesion, spreading, nucleus size, and vinculin expression. This high-throughput screening system enables elucidation of the relationships between biointerface properties and biological behavior, and thereby serves a potential tool for accelerating the development of high-performance biomaterials.

Original languageEnglish
Article number1800504
Number of pages8
JournalAdvanced Materials Interfaces
Volume5
Issue number18
DOIs
Publication statusPublished - 21-Sep-2018

Keywords

  • high-throughput screening
  • orthogonal double gradient
  • stem cell
  • stiffness
  • wettability
  • CHEMICAL GRADIENTS
  • CONTACT GUIDANCE
  • POLYMER SURFACES
  • ADHESION
  • DIFFERENTIATION
  • SUBSTRATE
  • STIFFNESS
  • MATRIX
  • MICROENVIRONMENTS
  • BIOMATERIALS

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