Development of cholesterol imprinted polymer-based interfaces as smart sensors for detection of cholesterol in clinical samples

Hunza Hayat, Fazli Rabbi Awan*, Ammara Aziz, Romana Schirhagl, Adeel Afzal, Adnan Mujahid, Arifa Jamil, Tayyaba Asim, Waheed S. Khan, Sadia Z. Bajwa*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Molecularly imprinted polymers (MIP) based on biomimetic approaches have proven their capabilities for creating highly specific receptor materials. We developed and investigated a biosensor based on MIP for the selective detection of cholesterol in synthetic media as well as in human blood sera. The surface roughness of imprinted interface increased to 4 nm as compared to non-imprinted indicating the presence of cholesterol-specific cavities. The concentration of cholesterol was related to the flow of charge across the interface of microchip. The biosensor showed a good linear relationship with the concentration of cholesterol ranging from 0.01 to 1 mM with a low limit of detection as 0.31 mM. The interface specifically recognized cholesterol three times as compared to cholecalciferol. The average clinical recovery rate of 98.81% indicates promising potential for an alternate to enzymatic biosensors for the determination of cholesterol. Graphical abstract: (Figure presented.)

Original languageEnglish
Pages (from-to)459-470
Number of pages12
JournalJournal of materials research
Volume39
Issue number3
DOIs
Publication statusPublished - 14-Feb-2024

Keywords

  • Biomarkers
  • Cardiovascular diseases
  • Cholesterol
  • Microchip
  • Molecular imprinting

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