Development of small-volume, microfluidic chaotic mixers for future application in two-dimensional liquid chromatography

Margaryta A. Ianovska, Patty P. M. F. A. Mulder, Elisabeth Verpoorte*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)
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Abstract

We report a microfluidic chaotic micromixer with staggered herringbone grooves having a geometry optimized for fast mobile-phase modification at the interface of a two-dimensional liquid chromatography system. The volume of the 300 mm mixers is 1.6 microliters and they provide mixing within 26 s at a flow rate of 4 mL min(-1) and 0.09 s at a flow rate of 1000 mL min(-1). Complete mixing is achieved within a distance of 3 cm along the 5 cm-long microchannel over the whole range of flow rates. The mixers can be used to mix aqueous phosphate-buffered saline solutions with methanol or acetonitrile at different ratios (1 : 2, 1 : 5 and 1 : 10). We also describe in detail an improved fabrication protocol for these mixers using a two-step soft photolithographic procedure. Mixers are made by replication in poly(dimethylsiloxane).

Original languageEnglish
Pages (from-to)9090-9099
Number of pages10
JournalRSC Advances
Volume7
Issue number15
DOIs
Publication statusPublished - 2017

Keywords

  • CIRCULATING TUMOR-CELLS
  • GROOVED MICROMIXERS
  • MICROCHANNELS
  • OPTIMIZATION
  • SIMULATION
  • MICROVORTEX
  • DILUTION
  • CAPTURE
  • DESIGN
  • FLOWS

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