Abstract
An evaluation is provided of dialysis, coupled on-line to column liquid chromatography, as a sample pretreatment procedure for macromolecule-containing biological samples. The influence of parameters such as acceptor phase flow rate, temperature, hydrophobicity of the analytes, pH, ionic strength and viscosity of the sample on the recovery and rate of dialysis is studied. In addition, methods to reduce the degree of drug-protein binding and thereby improve the recovery are reported. Diazepam, nitrazepam and oxazepam are used as model compounds. A method is reported for the fully automated determination of these compounds in human plasma using only 100 microliters of sample. Data on repeatability, linearity and detectability are given.
| Original language | English |
|---|---|
| Pages (from-to) | 225-33 |
| Number of pages | 9 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 10 |
| Issue number | 2-3 |
| Publication status | Published - 1-Feb-1992 |
| Externally published | Yes |
Keywords
- Anti-Anxiety Agents
- Blood Proteins
- Chromatography, Liquid
- Dialysis
- Diazepam
- Humans
- Hydrogen-Ion Concentration
- Nitrazepam
- Osmolar Concentration
- Oxazepam
- Protein Binding
- Temperature
- Viscosity