Differential expression of sphingolipids in P-glycoprotein or multidrug resistance-related protein 1 expressing human neuroblastoma cell lines

  • AJ Dijkhuis
  • , J Douwes
  • , W Kamps
  • , H Sietsma
  • , JW Kok*
  • *Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    18 Citations (Scopus)

    Abstract

    The sphingolipid composition and multidrug resistance status of three human neuroblastoma cell lines were established. SK-N-FI cells displayed high expression and functional (efflux) activity of P-glycoprotein, while multidrug resistance-related protein 1 was relatively abundant and most active in SK-N-AS cells. These two cell lines exhibited higher sphingolipid levels, compared to SK-N-DZ, which had the lowest activity of either ATP-binding cassette transporter protein. SK-N-DZ cells also differed in ganglioside composition with predominant expression of b-series gangliosides. In conclusion, these three neuroblastoma cell lines offer a good model system to study sphingolipid metabolism in relation to ATP-binding cassette transporter protein function. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)28-32
    Number of pages5
    JournalFEBS Letters
    Volume548
    Issue number1-3
    DOIs
    Publication statusPublished - 31-Jul-2003

    Keywords

    • neuroblastoma
    • sphingolipid
    • ganglioside
    • multidrug resistance
    • P-glycoprotein
    • multidrug resistance-related protein 1
    • CANCER-CELLS
    • CERAMIDE
    • GLUCOSYLCERAMIDE
    • METABOLISM
    • LEUKEMIA
    • PATTERN

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