Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution

C E Klostermann, P L Buwalda, H Leemhuis, P de Vos, H A Schols, J H Bitter*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
30 Downloads (Pure)

Abstract

Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A > B), followed by molecular weight (Mw) (high DP > low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

Original languageEnglish
Article number118069
Number of pages10
JournalCarbohydrate Polymers
Volume265
DOIs
Publication statusPublished - 1-Aug-2021

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