Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution

C E Klostermann; P L Buwalda; H Leemhuis; P de Vos; H A Schols; J H Bitter

doi:10.1016/j.carbpol.2021.118069

Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution

C E Klostermann, P L Buwalda, H Leemhuis, P de Vos, H A Schols, J H Bitter^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A > B), followed by molecular weight (Mw) (high DP > low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

Original language	English
Article number	118069
Number of pages	10
Journal	Carbohydrate Polymers
Volume	265
DOIs	https://doi.org/10.1016/j.carbpol.2021.118069
Publication status	Published - 1-Aug-2021

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title = "Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution",

abstract = "Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A > B), followed by molecular weight (Mw) (high DP > low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.",

author = "Klostermann, {C E} and Buwalda, {P L} and H Leemhuis and {de Vos}, P and Schols, {H A} and Bitter, {J H}",

year = "2021",

month = aug,

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doi = "10.1016/j.carbpol.2021.118069",

language = "English",

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TY - JOUR

T1 - Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution

AU - Klostermann, C E

AU - Buwalda, P L

AU - Leemhuis, H

AU - de Vos, P

AU - Schols, H A

AU - Bitter, J H

PY - 2021/8/1

Y1 - 2021/8/1

N2 - Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A > B), followed by molecular weight (Mw) (high DP > low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

AB - Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A > B), followed by molecular weight (Mw) (high DP > low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

U2 - 10.1016/j.carbpol.2021.118069

DO - 10.1016/j.carbpol.2021.118069

M3 - Article

C2 - 33966833

SN - 0144-8617

VL - 265

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

M1 - 118069

ER -

Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution

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