Directed Evolution of Enzymes: Library Screening Strategies

Hans Leemhuis; Ronan M. Kelly; Lubbert Dijkhuizen

doi:10.1002/iub.165

Directed Evolution of Enzymes: Library Screening Strategies

Hans Leemhuis, Ronan M. Kelly, Lubbert Dijkhuizen^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

87 Citations (Scopus)

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Abstract

Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. (C) 2009 IUBMB

Original language	English
Pages (from-to)	222-228
Number of pages	7
Journal	Iubmb Life
Volume	61
Issue number	3
DOIs	https://doi.org/10.1002/iub.165
Publication status	Published - Mar-2009

Keywords

cell surface display
DNA shuffling
enzymology
error-prone PCR
high-throughput
in vitro compartmentalization
protein engineering
screening
saturation mutagenesis
CYCLODEXTRIN GLUCANOTRANSFERASE
CATALYTIC PROMISCUITY
BETA-GALACTOSIDASE
IN-VITRO
DNA
ENANTIOSELECTIVITY
BIOCATALYSIS
COMPARTMENTS
ELIMINATION
MUTATIONS

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@article{c4a0c55cb6b849feb3826ed6fce0a84f,

title = "Directed Evolution of Enzymes: Library Screening Strategies",

abstract = "Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. (C) 2009 IUBMB",

keywords = "cell surface display, DNA shuffling, enzymology, error-prone PCR, high-throughput, in vitro compartmentalization, protein engineering, screening, saturation mutagenesis, CYCLODEXTRIN GLUCANOTRANSFERASE, CATALYTIC PROMISCUITY, BETA-GALACTOSIDASE, IN-VITRO, DNA, ENANTIOSELECTIVITY, BIOCATALYSIS, COMPARTMENTS, ELIMINATION, MUTATIONS",

author = "Hans Leemhuis and Kelly, {Ronan M.} and Lubbert Dijkhuizen",

year = "2009",

month = mar,

doi = "10.1002/iub.165",

language = "English",

volume = "61",

pages = "222--228",

journal = "Iubmb Life",

issn = "1521-6543",

number = "3",

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TY - JOUR

T1 - Directed Evolution of Enzymes

T2 - Library Screening Strategies

AU - Leemhuis, Hans

AU - Kelly, Ronan M.

AU - Dijkhuizen, Lubbert

PY - 2009/3

Y1 - 2009/3

N2 - Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. (C) 2009 IUBMB

AB - Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. (C) 2009 IUBMB

KW - cell surface display

KW - DNA shuffling

KW - enzymology

KW - error-prone PCR

KW - high-throughput

KW - in vitro compartmentalization

KW - protein engineering

KW - screening

KW - saturation mutagenesis

KW - CYCLODEXTRIN GLUCANOTRANSFERASE

KW - CATALYTIC PROMISCUITY

KW - BETA-GALACTOSIDASE

KW - IN-VITRO

KW - DNA

KW - ENANTIOSELECTIVITY

KW - BIOCATALYSIS

KW - COMPARTMENTS

KW - ELIMINATION

KW - MUTATIONS

U2 - 10.1002/iub.165

DO - 10.1002/iub.165

M3 - Review article

SN - 1521-6543

VL - 61

SP - 222

EP - 228

JO - Iubmb Life

JF - Iubmb Life

IS - 3

ER -

Directed Evolution of Enzymes: Library Screening Strategies

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Keywords

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