Directed evolution of epoxide hydrolase from A-radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling

B van Loo; Jeffrey Lutje Spelberg; J Kingma; T. Sonke; Marcel Wubbolts; D.B. Janssen

doi:10.1016/j.chembiol.2004.04.019

Directed evolution of epoxide hydrolase from A-radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling

B van Loo, Jeffrey Lutje Spelberg, J Kingma, T. Sonke, Marcel Wubbolts, D.B. Janssen

Research output: Contribution to journal › Article › Academic › peer-review

114 Citations (Scopus)

412 Downloads (Pure)

Original language	English
Pages (from-to)	981-990
Number of pages	10
Journal	Chemistry & Biology
Volume	11
Issue number	7
DOIs	https://doi.org/10.1016/j.chembiol.2004.04.019
Publication status	Published - 2004

Keywords

ALKYLATION HALF-REACTION
AGROBACTERIUM-RADIOBACTER
HALOALKANE DEHALOGENASE
CATALYTIC MECHANISM
KINETIC RESOLUTION
NITROSTYRENE OXIDE
ASPERGILLUS-NIGER
STYRENE OXIDE
HYDROLYSIS
AD1

Access to Document

10.1016/j.chembiol.2004.04.019

2004ChemBiolvLoo.pdfFinal publisher's version, 278 KB

Handle.net

Persistent link

Cite this

@article{3fc66fd4773a47149e9474390fa86f01,

title = "Directed evolution of epoxide hydrolase from A-radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling",

keywords = "ALKYLATION HALF-REACTION, AGROBACTERIUM-RADIOBACTER, HALOALKANE DEHALOGENASE, CATALYTIC MECHANISM, KINETIC RESOLUTION, NITROSTYRENE OXIDE, ASPERGILLUS-NIGER, STYRENE OXIDE, HYDROLYSIS, AD1",

author = "{van Loo}, B and {Lutje Spelberg}, Jeffrey and J Kingma and T. Sonke and Marcel Wubbolts and D.B. Janssen",

note = "J UL",

year = "2004",

doi = "10.1016/j.chembiol.2004.04.019",

language = "English",

volume = "11",

pages = "981--990",

journal = "Chemistry & Biology",

issn = "1074-5521",

publisher = "Cell Press",

number = "7",

}

TY - JOUR

T1 - Directed evolution of epoxide hydrolase from A-radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling

AU - van Loo, B

AU - Lutje Spelberg, Jeffrey

AU - Kingma, J

AU - Sonke, T.

AU - Wubbolts, Marcel

AU - Janssen, D.B.

N1 - J UL

PY - 2004

Y1 - 2004

KW - ALKYLATION HALF-REACTION

KW - AGROBACTERIUM-RADIOBACTER

KW - HALOALKANE DEHALOGENASE

KW - CATALYTIC MECHANISM

KW - KINETIC RESOLUTION

KW - NITROSTYRENE OXIDE

KW - ASPERGILLUS-NIGER

KW - STYRENE OXIDE

KW - HYDROLYSIS

KW - AD1

U2 - 10.1016/j.chembiol.2004.04.019

DO - 10.1016/j.chembiol.2004.04.019

M3 - Article

SN - 1074-5521

VL - 11

SP - 981

EP - 990

JO - Chemistry & Biology

JF - Chemistry & Biology

IS - 7

ER -