Directional topography gradients drive optimum alignment and differentiation of human myoblasts

Ana Maria Almonacid Suarez, Qihui Zhou, Patrick van Rijn*, Martin C. Harmsen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
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Abstract

Tissue engineering of skeletal muscle aims to replicate the parallel alignment of myotubes on the native tissue. Directional topography gradients allow the study of the influence of topography on cellular orientation, proliferation, and differentiation, resulting in yield cues and clues to develop a proper in vitro environment for muscle tissue engineering. In this study, we used a polydimethylsiloxane-based substrate containing an aligned topography gradient with sinusoidal features ranging from wavelength (lambda) = 1,520 nm and amplitude (A) =176 nm to lambda = 9,934 nm and A = 2,168 nm. With this topography gradient, we evaluated the effect of topography on human myoblasts distribution, dominant orientation, cell area, nuclei coverage, cell area per number of nuclei, and nuclei area of myotubes. We showed that human myoblasts aligned and differentiated irrespective of the topography section. In addition, aligned human myotubes showed functionality and maturity by contracting spontaneously and nuclei peripheral organization resembling natural myotubes.

Original languageEnglish
Number of pages12
JournalJournal of tissue engineering and regenerative medicine
Early online date1-Nov-2019
DOIs
Publication statusPublished - 10-Nov-2019

Keywords

  • myoblasts
  • myotubes
  • polydimethylsiloxane (PDMS)
  • tissue engineering
  • topography gradient
  • SCREENING PLATFORM
  • SATELLITE CELLS
  • MUSCLE
  • ORIENTATION
  • ORGANIZATION
  • PATTERNS
  • MYOTUBES
  • MODEL

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