Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells

Hilde M. van der Schaar*, Michael J. Rust, Chen Chen, Heidi van der Ende-Metselaar, Jan Wilschut, Xiaowei Zhuang, Jolanda M. Smit

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    317 Citations (Scopus)
    257 Downloads (Pure)

    Abstract

    Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and single-virus tracking to investigate the cell entry, endocytic trafficking, and fusion behavior of DENV. Simultaneous tracking of DENV particles and various endocytic markers revealed that DENV enters cells exclusively via clathrin-mediated endocytosis. The virus particles move along the cell surface in a diffusive manner before being captured by a pre-existing clathrin-coated pit. Upon clathrin-mediated entry, DENV particles are transported to Rab5-positive endosomes, which subsequently mature into late endosomes through acquisition of Rab7 and loss of Rab5. Fusion of the viral membrane with the endosomal membrane was primarily detected in late endosomal compartments.

    Original languageEnglish
    Article number1000244
    Number of pages9
    JournalPLoS Pathogens
    Volume4
    Issue number12
    DOIs
    Publication statusPublished - 19-Dec-2008

    Keywords

    • INDIVIDUAL INFLUENZA-VIRUSES
    • FLAVIVIRUS WEST-NILE
    • CLATHRIN-COATED PITS
    • VERO CELLS
    • JAPANESE ENCEPHALITIS
    • ENDOCYTIC MACHINERY
    • INFECTIOUS ENTRY
    • ENVELOPE PROTEIN
    • MEMBRANE-FUSION
    • MOSQUITO CELLS

    Cite this