TY - UNPB
T1 - Dissection of purified LINE-1 reveals distinct nuclear and cytoplasmic intermediates
AU - Molloy, K.R.
AU - Taylor, M.S.
AU - Altukhov, I.
AU - Mita, P.
AU - Jiang, H.
AU - Adney, E.M.
AU - Wudzinska, A.
AU - Ischenko, D.
AU - Burns, K.H.
AU - Fenyö, D.
AU - Chait, B.T.
AU - Alexeev, D.
AU - Rout, M.P.
AU - Boeke, J.D.
AU - LaCava, J.
PY - 2017
Y1 - 2017
N2 - Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications and quantitative mass spectrometry, we have characterized the proteins associated with distinctive L1 macromolecular complexes. Our findings support the presence of multiple L1-derived retrotransposition intermediates in vivo. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in template-primed reverse transcription.
AB - Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications and quantitative mass spectrometry, we have characterized the proteins associated with distinctive L1 macromolecular complexes. Our findings support the presence of multiple L1-derived retrotransposition intermediates in vivo. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNA-containing RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in template-primed reverse transcription.
U2 - 10.1101/157818
DO - 10.1101/157818
M3 - Preprint
T3 - bioRxiv
BT - Dissection of purified LINE-1 reveals distinct nuclear and cytoplasmic intermediates
PB - BioRxiv
ER -