Distinction of Staphylococcal Cassette Chromosome mec type V elements from Staphylococcus aureus ST398

Monika A. Chlebowicz, Thijs Bosch, Artur J. Sabat, Jan Paul Arends, Hajo Grundmann, Jan Maarten van Dijl*, Girbe Buist

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Methicillin resistant S. aureus (MRSA) is a major threat for human health and well-being. In recent years, it has become clear that livestock is a potential reservoir for MRSA, most livestock-associated isolates belonging to the ST398 lineage. Importantly, ST398 strains were also reported as causative agents of severe invasive infections in humans with no evidence for livestock associations. Here we document the sequence of the J1 region of the type V (5C2&5) SCCmec element and its right chromosomal junction in the clinical PVL-positive ST398 MRSA isolate UMCG-M4. Sequence comparisons show that this SCCmec element and related type V elements from other S. aureus isolates share a common core structure, but differ substantially in the so-called J1 region. Additional PCR analyses and typing studies indicate that the J1 region of strain UMCG-M4 is specific for SCCmec elements of PVL-positive ST398 isolates. Lastly, we show that the sequenced right chromosomal junction is invariant in strains of the ST398 lineage. (C) 2013 Elsevier GmbH. All rights reserved.

Original languageEnglish
Pages (from-to)422-432
Number of pages11
JournalInternational journal of medical microbiology
Volume303
Issue number8
DOIs
Publication statusPublished - Dec-2013

Keywords

  • SCCmec
  • Type V
  • 5C2&5
  • ST398
  • Chromosomal junction
  • J1 region
  • MRSA
  • FIELD GEL-ELECTROPHORESIS
  • TANDEM REPEAT ANALYSIS
  • METHICILLIN RESISTANCE
  • TYPING METHODS
  • SCCMEC TYPES
  • STRAINS
  • GENOME
  • DIVERSITY
  • EVOLUTION
  • SEQUENCE

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