DISTRIBUTION OF HEARTWATER IN THE CARIBBEAN DETERMINED ON THE BASIS OF DETECTION OF ANTIBODIES TO THE CONSERVED 32-KILODALTON PROTEIN OF COWDRIA-RUMINANTIUM

A.C. Muller Kobold; D MARTINEZ; E CAMUS; F JONGEJAN

DISTRIBUTION OF HEARTWATER IN THE CARIBBEAN DETERMINED ON THE BASIS OF DETECTION OF ANTIBODIES TO THE CONSERVED 32-KILODALTON PROTEIN OF COWDRIA-RUMINANTIUM

A.C. Muller Kobold, D MARTINEZ, E CAMUS, F JONGEJAN

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

15 Citations (Scopus)

Abstract

A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies to the major 32-kDa protein of Cowdria ruminantium. A total of 1,804 serum samples collected from cattle on 19 islands in the eastern Caribbean Basin were tested by this cELISA. A total of 133 serum samples from 10 islands (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, St. Lucia, St. Martin, and St. Vincent) were found to be positive. The presence of antibodies to C. ruminantium in cattle on these islands was confirmed by immunofluorescence and Western blotting (immunoblotting). In earlier studies, C. ruminantium has been demonstrated only on Guadeloupe, Antigua, and Marie Galante. This study shows that the causative agent of heartwater is now firmly established in the Caribbean.

Original language	English
Pages (from-to)	1870-1873
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	30
Issue number	7
Publication status	Published - Jul-1992
Externally published	Yes

Keywords

IDENTIFICATION
STOCKS

Handle.net

Persistent link

Cite this

@article{4edad51624fc46d3be23c7bd7b597c0d,

title = "DISTRIBUTION OF HEARTWATER IN THE CARIBBEAN DETERMINED ON THE BASIS OF DETECTION OF ANTIBODIES TO THE CONSERVED 32-KILODALTON PROTEIN OF COWDRIA-RUMINANTIUM",

abstract = "A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies to the major 32-kDa protein of Cowdria ruminantium. A total of 1,804 serum samples collected from cattle on 19 islands in the eastern Caribbean Basin were tested by this cELISA. A total of 133 serum samples from 10 islands (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, St. Lucia, St. Martin, and St. Vincent) were found to be positive. The presence of antibodies to C. ruminantium in cattle on these islands was confirmed by immunofluorescence and Western blotting (immunoblotting). In earlier studies, C. ruminantium has been demonstrated only on Guadeloupe, Antigua, and Marie Galante. This study shows that the causative agent of heartwater is now firmly established in the Caribbean.",

keywords = "IDENTIFICATION, STOCKS",

author = "{Muller Kobold}, A.C. and D MARTINEZ and E CAMUS and F JONGEJAN",

year = "1992",

month = jul,

language = "English",

volume = "30",

pages = "1870--1873",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "AMER SOC MICROBIOLOGY",

number = "7",

}

DISTRIBUTION OF HEARTWATER IN THE CARIBBEAN DETERMINED ON THE BASIS OF DETECTION OF ANTIBODIES TO THE CONSERVED 32-KILODALTON PROTEIN OF COWDRIA-RUMINANTIUM. / Muller Kobold, A.C.; MARTINEZ, D; CAMUS, E et al.
In: Journal of Clinical Microbiology, Vol. 30, No. 7, 07.1992, p. 1870-1873.

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

TY - JOUR

T1 - DISTRIBUTION OF HEARTWATER IN THE CARIBBEAN DETERMINED ON THE BASIS OF DETECTION OF ANTIBODIES TO THE CONSERVED 32-KILODALTON PROTEIN OF COWDRIA-RUMINANTIUM

AU - Muller Kobold, A.C.

AU - MARTINEZ, D

AU - CAMUS, E

AU - JONGEJAN, F

PY - 1992/7

Y1 - 1992/7

N2 - A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies to the major 32-kDa protein of Cowdria ruminantium. A total of 1,804 serum samples collected from cattle on 19 islands in the eastern Caribbean Basin were tested by this cELISA. A total of 133 serum samples from 10 islands (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, St. Lucia, St. Martin, and St. Vincent) were found to be positive. The presence of antibodies to C. ruminantium in cattle on these islands was confirmed by immunofluorescence and Western blotting (immunoblotting). In earlier studies, C. ruminantium has been demonstrated only on Guadeloupe, Antigua, and Marie Galante. This study shows that the causative agent of heartwater is now firmly established in the Caribbean.

AB - A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies to the major 32-kDa protein of Cowdria ruminantium. A total of 1,804 serum samples collected from cattle on 19 islands in the eastern Caribbean Basin were tested by this cELISA. A total of 133 serum samples from 10 islands (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, St. Lucia, St. Martin, and St. Vincent) were found to be positive. The presence of antibodies to C. ruminantium in cattle on these islands was confirmed by immunofluorescence and Western blotting (immunoblotting). In earlier studies, C. ruminantium has been demonstrated only on Guadeloupe, Antigua, and Marie Galante. This study shows that the causative agent of heartwater is now firmly established in the Caribbean.

KW - IDENTIFICATION

KW - STOCKS

M3 - Comment/Letter to the editor

SN - 0095-1137

VL - 30

SP - 1870

EP - 1873

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

DISTRIBUTION OF HEARTWATER IN THE CARIBBEAN DETERMINED ON THE BASIS OF DETECTION OF ANTIBODIES TO THE CONSERVED 32-KILODALTON PROTEIN OF COWDRIA-RUMINANTIUM

Abstract

Keywords

Handle.net

Fingerprint

Cite this