DNA methylation and body mass index from birth to adolescence: meta-analyses of epigenome-wide association studies

Florianne O. L. Vehmeijer, Leanne K. Kuepers, Gemma C. Sharp, Lucas A. Salas, Samantha Lent, Dereje D. Jima, Gwen Tindula, Sarah Reese, Cancan Qi, Olena Gruzieva, Christian Page, Faisal Rezwan, Philip E. Melton, Ellen Nohr, Georgia Escaramis, Peter Rzehak, Anni Heiskala, Tong Gong, Samuli T. Tuominen, Lu GaoJason P. Ross, Anne P. Starling, John W. Holloway, Paul Yousefi, Gunn Marit Aasvang, Lawrence J. Beilin, Anna Bergstrom, Elisabeth Binder, Leda Chatzi, Eva Corpeleijn, Darina Czamara, Brenda Eskenazi, Susan Ewart, Natalia Ferre, Veit Grote, Dariusz Gruszfeld, Siri E. Haberg, Cathrine Hoyo, Karen Huen, Robert Karlsson, Inger Kull, Jean-Paul Langhendries, Johanna Lepeule, Maria C. Magnus, Rachel L. Maguire, Peter L. Molloy, Claire Monnereau, Trevor A. Mori, Emily Oken, Katri Raikkonen, Sheryl Rifas-Shiman, Carlos Ruiz-Arenas, Sylvain Sebert, Vilhelmina Ullemar, Elvira Verduci, Judith M. Vonk, Cheng-jian Xu, Ivana Yang, Hongmei Zhang, Weiming Zhang, Wilfried Karmaus, Dana Dabelea, Beverly S. Muhlhausler, Carrie Breton, Jari Lahti, Catarina Almqvist, Marjo-Riitta Jarvelin, Berthold Koletzko, Martine Vrijheid, Thorkild I. A. Sorensen, Rae-Chi Huang, Syed Hasan Arshad, Wenche Nystad, Erik Melen, Gerard H. Koppelman, Stephanie J. London, Nina Holland, Mariona Bustamante, Susan K. Murphy, Marie-France Hivert, Andrea Baccarelli, Caroline L. Relton, Harold Snieder, Vincent W. V. Jaddoe, Janine F. Felix*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

BACKGROUND: DNA methylation has been shown to be associated with adiposity in adulthood. However, whether similar DNA methylation patterns are associated with childhood and adolescent body mass index (BMI) is largely unknown. More insight into this relationship at younger ages may have implications for future prevention of obesity and its related traits.

METHODS: We examined whether DNA methylation in cord blood and whole blood in childhood and adolescence was associated with BMI in the age range from 2 to 18 years using both cross-sectional and longitudinal models. We performed meta-analyses of epigenome-wide association studies including up to 4133 children from 23 studies. We examined the overlap of findings reported in previous studies in children and adults with those in our analyses and calculated enrichment.

RESULTS: DNA methylation at three CpGs (cg05937453, cg25212453, and cg10040131), each in a different age range, was associated with BMI at Bonferroni significance, P < 1.06 × 10-7, with a 0.96 standard deviation score (SDS) (standard error (SE) 0.17), 0.32 SDS (SE 0.06), and 0.32 BMI SDS (SE 0.06) higher BMI per 10% increase in methylation, respectively. DNA methylation at nine additional CpGs in the cross-sectional childhood model was associated with BMI at false discovery rate significance. The strength of the associations of DNA methylation at the 187 CpGs previously identified to be associated with adult BMI, increased with advancing age across childhood and adolescence in our analyses. In addition, correlation coefficients between effect estimates for those CpGs in adults and in children and adolescents also increased. Among the top findings for each age range, we observed increasing enrichment for the CpGs that were previously identified in adults (birth Penrichment = 1; childhood Penrichment = 2.00 × 10-4; adolescence Penrichment = 2.10 × 10-7).

CONCLUSIONS: There were only minimal associations of DNA methylation with childhood and adolescent BMI. With the advancing age of the participants across childhood and adolescence, we observed increasing overlap with altered DNA methylation loci reported in association with adult BMI. These findings may be compatible with the hypothesis that DNA methylation differences are mostly a consequence rather than a cause of obesity.

Original languageEnglish
Article number105
Number of pages15
JournalGenome medicine
Volume12
Issue number1
DOIs
Publication statusPublished - 25-Dec-2020

Keywords

  • Body mass index
  • Childhood obesity
  • DNA methylation
  • Epigenetics
  • CORD BLOOD
  • PROMOTER METHYLATION
  • LMS METHOD
  • OBESITY
  • ADIPOSITY
  • CHILDHOOD
  • DISCOVERY
  • CHILDREN
  • LOCI
  • BMI

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