Abstract
Background: We examined whether the effect of maternal smoking during pregnancy on birthweight of the offspring was mediated by smoking-induced changes to DNA methylation in cord blood.
Methods: First, we used cord blood of 129 Dutch children exposed to maternal smoking vs 126 unexposed to maternal and paternal smoking (53% male) participating in the GECKO Drenthe birth cohort. DNA methylation was measured using the Illumina HumanMethylation450 Beadchip. We performed an epigenome-wide association study for the association between maternal smoking and methylation followed by a mediation analysis of the top signals [false-discovery rate (FDR)
Results: We found 35 differentially methylated CpGs (FDR
Conclusions: Maternal smoking during pregnancy was associated with cord blood methylation differences. We observed a potentially mediating role of methylation in the association between maternal smoking during pregnancy and birthweight of the offspring. Functional network analysis suggested a role in activating the immune system.
Original language | English |
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Pages (from-to) | 1224-1237 |
Number of pages | 14 |
Journal | International Journal of Epidemiology |
Volume | 44 |
Issue number | 4 |
DOIs | |
Publication status | Published - Aug-2015 |
Keywords
- Epigenetic epidemiology
- epigenome-wide association study
- DOHaD
- fetal programming
- GECKO
- ALSPAC
- Generation R
- GENE-EXPRESSION
- PRESCHOOL-CHILDREN
- TOBACCO-SMOKE
- CORD BLOOD
- EXPOSURE
- COHORT
- ASSOCIATION
- RISK
- IDENTIFICATION
- AUTOIMMUNITY
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GECKO Drenthe cohort
Corpeleijn, E. (Creator), Snieder, H. (Creator), Bocca, G. (Creator), Gracchi, V. (Creator) & Hoek, A. (Creator), University of Groningen, 2006
DOI: 10.34760/5f5b80eb0830f, http://www.geckodrenthe.umcg.nl and 2 more links, http://www.birthcohorts.net/birthcohorts/birthcohort/?id=138, https://umcgresearch.org/-/gecko (show fewer)
Dataset