Domain organization of photosystem II in membranes of the cyanobacterium Synechocystis PCC6803 investigated by electron microscopy

I. Mihaela Folea, Pengpeng Zhang, Eva-Mari Aro, Egbert J. Boekema*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The supramolecular organization of photosystem II (PSII) complexes in the photosynthetic membrane of the cyanobacterium Synechocystis 6803 was studied by electron microscopy. After mild detergent solubilization, crystalline PSII arrays were extracted in which dimeric PSII particles associate in multiple rows. Image processing of the arrays shows that the PSII dimers are tightly packed at distances of 12.2 and 16.7 nm. The domains are considered to be an important type of association for preventing either spill-over energy from PSII towards photosystem I (PSI) or direct energy flow from phycobilisomes to PSI, because the latter can only be at periphery of the arrays. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)1749-1754
Number of pages6
JournalFEBS Letters
Volume582
Issue number12
DOIs
Publication statusPublished - 28-May-2008

Keywords

  • electron microscopy
  • photosystem II
  • single particle analysis
  • membrane organization
  • Synechocystis 6803
  • THYLAKOID MEMBRANES
  • SUPRAMOLECULAR ORGANIZATION
  • ANGSTROM RESOLUTION
  • STATE TRANSITIONS
  • SYNECHOCOCCUS SP
  • PHYCOBILISOME
  • COMPLEXES
  • PCC-6803
  • STOICHIOMETRY
  • PROTEINS

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