Double-strand breaks are not the main cause of spontaneous sister chromatid exchange in wild-type yeast cells

Clemence Claussin, David Porubský, Diana C. J. Spierings, Nancy Halsema, Stefan Rentas, Victor Guryev, Peter M. Lansdorp, Michael Chang

Research output: Contribution to journalArticleAcademic

Abstract

Homologous recombination involving sister chromatids is the most accurate, and thus most frequently used, form of recombination-mediated DNA repair. Despite its importance, sister chromatid recombination is not easily studied because it does not result in a change in DNA sequence, making recombination between sister chromatids difficult to detect. We have previously developed a novel DNA template strand sequencing technique, called Strand-seq, that can be used to map sister chromatid exchange (SCE) events genome-wide in single cells. An increase in the rate of SCE is an indicator of elevated recombination activity and of genome instability, which is a hallmark of cancer. In this study, we have adapted Strand-seq to detect SCE in the yeast Saccharomyces cerevisiae. Contrary to what is commonly thought, we find that most spontaneous SCE events are not due to the repair of DNA double-strand breaks.
Original languageEnglish
Number of pages36
JournalbioRxiv
DOIs
Publication statusE-pub ahead of print - 17-Jul-2017

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