During viral infection of the respiratory tract, CD27, 4-1BB, and OX40 collectively determine formation of CD8+ memory T cells and their capacity for secondary expansion

Jenny Hendriks, Yanling Xiao, John W A Rossen, Koenraad F van der Sluijs, Kazuo Sugamura, Naoto Ishii, Jannie Borst

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    170 Citations (Scopus)

    Abstract

    Independent studies have shown that CD27, 4-1BB, and OX40 can all promote survival of activated CD8+ T cells. We have therefore compared their impact on CD8+ memory T cell formation and responsiveness within one, physiologically relevant model system. Recombinant mice, selectively lacking input of one or two receptors, were challenged intranasally with influenza virus, and the immunodominant virus-specific CD8+ T cell response was quantified at priming and effector sites. Upon primary infection, CD27 and (to a lesser extent) 4-1BB made nonredundant contributions to accumulation of CD8+ virus-specific T cells in draining lymph nodes and lung, while OX40 had no effect. Interestingly though, in the memory response, accumulation of virus-specific CD8+ T cells in spleen and lung critically depended on all three receptor systems. This was explained by two observations: 1) CD27, 4-1BB, and OX40 were collectively responsible for generation of the same memory CD8+ T cell pool; 2) CD27, 4-1BB, and OX40 collectively determined the extent of secondary expansion, as shown by adoptive transfers with standardized numbers of memory cells. Surprisingly, wild-type CD8+ memory T cells expanded normally in primed OX40 ligand- or 4-1BB ligand-deficient mice. However, when wild-type memory cells were generated in OX40 ligand- or 4-1BB ligand-deficient mice, their secondary expansion was impaired. This provides the novel concept that stimulation of CD8+ T cells by OX40 and 4-1BB ligand during priming imprints into them the capacity for secondary expansion. Our data argue that ligand on dendritic cells and/or B cells may be critical for this.

    Original languageEnglish
    Pages (from-to)1665-1676
    Number of pages12
    JournalJournal of Immunology
    Volume175
    Issue number3
    Publication statusPublished - 1-Aug-2005

    Keywords

    • Administration, Intranasal
    • Adoptive Transfer
    • Animals
    • Antibodies, Viral
    • Antigen-Presenting Cells
    • Antigens, CD
    • Antigens, CD137
    • Antigens, CD27
    • CD8-Positive T-Lymphocytes
    • Cell Proliferation
    • Genomic Imprinting
    • Immunization, Secondary
    • Immunologic Memory
    • Influenza A virus
    • Ligands
    • Lymphocyte Activation
    • Membrane Glycoproteins
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Orthomyxoviridae Infections
    • Receptors, Nerve Growth Factor
    • Receptors, OX40
    • Receptors, Tumor Necrosis Factor
    • T-Lymphocyte Subsets
    • Tumor Necrosis Factors

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