eap gene as novel target for specific identification of Staphylococcus aureus

Muzaffar Hussain, Christof von Eiff, Bhanu Sinha, Insa Joost, Mathias Herrmann, Georg Peters, Karsten Becker*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

The cell surface-associated extracellular adherence protein (Eap) mediates adherence of Staphylococcus aureus to host extracellular matrix components and inhibits inflammation, wound healing, and angiogenesis. A well-characterized collection of S. aureus and non-S. aureus staphylococcal isolates (n = 813) was tested for the presence of the Eap-encoding gene (eap) by PCR to investigate the use of the eap gene as a specific diagnostic too] for identification of S. aureus. Whereas all 597 S. aureus isolates were eap positive, this gene was not detectable in 216 non-S. aureus staphylococcal isolates comprising 47 different species and subspecies of coagulase-negative staphylococci and non-S. aureus coagulase-positive or coagulase-variable staphylococci. Furthermore, non-S. aureus isolates did not express Eap homologs, as verified on the transcriptional and protein levels. Based on these data, the sensitivity and specificity of the newly developed PCR targeting the eap gene were both 100%. Thus, the unique occurrence of Eap in S. aureus offers a promising tool particularly suitable for molecular diagnostics of this pathogen.

Original languageEnglish
Pages (from-to)470-476
Number of pages7
JournalJournal of Clinical Microbiology
Volume46
Issue number2
DOIs
Publication statusPublished - Feb-2008
Externally publishedYes

Keywords

  • POLYMERASE-CHAIN-REACTION
  • EXTRACELLULAR ADHERENCE PROTEIN
  • MULTIPLEX PCR
  • THERMONUCLEASE GENE
  • ENTEROTOXIN GENES
  • HOST-DEFENSE
  • AMPLIFICATION
  • BINDING
  • MATRIX
  • CELLS

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