ECF-Type ATP-Binding Cassette Transporters

S Rempel, W K Stanek*, D J Slotboom

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)
135 Downloads (Pure)

Abstract

Energy-coupling factor (ECF)-type ATP-binding cassette (ABC) transporters catalyze membrane transport of micronutrients in prokaryotes. Crystal structures and biochemical characterization have revealed that ECF transporters are mechanistically distinct from other ABC transport systems. Notably, ECF transporters make use of small integral membrane subunits (S-components) that are predicted to topple over in the membrane when carrying the bound substrate from the extracellular side of the bilayer to the cytosol. Here, we review the phylogenetic diversity of ECF transporters as well as recent structural and biochemical advancements that have led to the postulation of conceptually different mechanistic models. These models can be described as power stroke and thermal ratchet. Structural data indicate that the lipid composition and bilayer structure are likely to have great impact on the transport function. We argue that study of ECF transporters could lead to generic insight of membrane protein structure, dynamics, and interaction. Expected final online publication date for the Annual Review of Biochemistry Volume 88 is June 20, 2019. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Original languageEnglish
Article numberannurev-biochem-013118-111705
Pages (from-to)551-576
Number of pages26
JournalAnnual Review of Biochemistry
Volume88
Early online date28-Nov-2018
DOIs
Publication statusPublished - Jun-2019

Keywords

  • ATP-binding cassette transporter
  • ABC transporter
  • energy-coupling factor type
  • ECF-type
  • prokaryotic vitamin uptake
  • membrane transport
  • mechanism of transport
  • ATP-driven transport
  • INDUCED CONFORMATIONAL-CHANGES
  • COUPLING FACTOR TRANSPORTER
  • CRYSTAL-STRUCTURE
  • ABC TRANSPORTERS
  • FOLATE TRANSPORT
  • S-COMPONENT
  • MALTOSE TRANSPORTER
  • LACTOCOCCUS-LACTIS
  • ESCHERICHIA-COLI
  • BIOTIN TRANSPORT

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