EfaR Is a Major Regulator of Enterococcus faecalis Manganese Transporters and Influences Processes Involved in Host Colonization and Infection

M. C. Abrantes, J. Kok*, M. de F. Lopes

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Metal ions, in particular manganese, are important modulators of bacterial pathogenicity. However, little is known about the role of manganese-dependent proteins in the nosocomial pathogen Enterococcus faecalis, a major cause of bacterial endocarditis. The present study demonstrates that the DtxR/MntR family metalloregulator EfaR of E. faecalis controls the expression of several of its regulon members in a manganese-dependent way. We also show that efaR inactivation impairs the ability of E. faecalis to form biofilms, to survive inside macrophages, and to tolerate oxidative stress. Our results reveal that EfaR is an important modulator of E. faecalis virulence and link manganese homeostasis to enterococcal pathogenicity.

Original languageEnglish
Pages (from-to)935-944
Number of pages10
JournalInfection and Immunity
Volume81
Issue number3
DOIs
Publication statusPublished - Mar-2013

Keywords

  • STREPTOCOCCUS-PNEUMONIAE
  • LACTOCOCCUS-LACTIS
  • ESCHERICHIA-COLI
  • GENE-EXPRESSION
  • TRACE-ELEMENTS
  • VIRULENCE
  • IRON
  • IDENTIFICATION
  • TRANSCRIPTION
  • MACROPHAGES

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