Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier

Nemanja Miletic, Zorica Vukovic, Aleksandra Nastasovic, Katja Loos*, Nemanja Miletić, Zorica Vuković, Aleksandra Nastasović

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.

Original languageEnglish
Pages (from-to)1537-1543
Number of pages7
JournalMacromolecular Bioscience
Volume11
Issue number11
DOIs
Publication statusPublished - 10-Nov-2011

Keywords

  • diffusion
  • enzymes
  • immobilization
  • lipase
  • macroporous copolymers
  • GLYCOL DIMETHACRYLATE) RESINS
  • GLYCIDYL METHACRYLATE
  • REACTIVE POLYMERS
  • ORGANIC-SOLVENTS
  • PORE-SIZE
  • COPOLYMERS
  • ENZYMES
  • ETHYLENEDIMETHACRYLATE
  • CHROMATOGRAPHY
  • INVERTASE

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