Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier

Nemanja Miletic; Zorica Vukovic; Aleksandra Nastasovic; Katja Loos; Nemanja Miletić; Zorica Vuković; Aleksandra Nastasović

doi:10.1002/mabi.201100127

Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier

Nemanja Miletic, Zorica Vukovic, Aleksandra Nastasovic, Katja Loos^*, Nemanja Miletić, Zorica Vuković, Aleksandra Nastasović

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

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Abstract

A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.

Original language	English
Pages (from-to)	1537-1543
Number of pages	7
Journal	Macromolecular Bioscience
Volume	11
Issue number	11
DOIs	https://doi.org/10.1002/mabi.201100127
Publication status	Published - 10-Nov-2011

Keywords

diffusion
enzymes
immobilization
lipase
macroporous copolymers
GLYCOL DIMETHACRYLATE) RESINS
GLYCIDYL METHACRYLATE
REACTIVE POLYMERS
ORGANIC-SOLVENTS
PORE-SIZE
COPOLYMERS
ENZYMES
ETHYLENEDIMETHACRYLATE
CHROMATOGRAPHY
INVERTASE

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Cite this

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title = "Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier",

abstract = "A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.",

keywords = "diffusion, enzymes, immobilization, lipase, macroporous copolymers, GLYCOL DIMETHACRYLATE) RESINS, GLYCIDYL METHACRYLATE, REACTIVE POLYMERS, ORGANIC-SOLVENTS, PORE-SIZE, COPOLYMERS, ENZYMES, ETHYLENEDIMETHACRYLATE, CHROMATOGRAPHY, INVERTASE",

author = "Nemanja Miletic and Zorica Vukovic and Aleksandra Nastasovic and Katja Loos and Nemanja Mileti{\'c} and Zorica Vukovi{\'c} and Aleksandra Nastasovi{\'c}",

year = "2011",

month = nov,

day = "10",

doi = "10.1002/mabi.201100127",

language = "English",

volume = "11",

pages = "1537--1543",

journal = "Macromolecular Bioscience",

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Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier. / Miletic, Nemanja; Vukovic, Zorica; Nastasovic, Aleksandra; Loos, Katja; Miletić, Nemanja; Vuković, Zorica; Nastasović, Aleksandra.

In: Macromolecular Bioscience, Vol. 11, No. 11, 10.11.2011, p. 1537-1543.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier

AU - Miletic, Nemanja

AU - Vukovic, Zorica

AU - Nastasovic, Aleksandra

AU - Loos, Katja

AU - Miletić, Nemanja

AU - Vuković, Zorica

AU - Nastasović, Aleksandra

PY - 2011/11/10

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N2 - A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.

AB - A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.

KW - diffusion

KW - enzymes

KW - immobilization

KW - lipase

KW - macroporous copolymers

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KW - GLYCIDYL METHACRYLATE

KW - REACTIVE POLYMERS

KW - ORGANIC-SOLVENTS

KW - PORE-SIZE

KW - COPOLYMERS

KW - ENZYMES

KW - ETHYLENEDIMETHACRYLATE

KW - CHROMATOGRAPHY

KW - INVERTASE

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DO - 10.1002/mabi.201100127

M3 - Article

VL - 11

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JO - Macromolecular Bioscience

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