Effect of ethylene oxide sterilization on enamel and dentin demineralization in vitro

Renske Z. Thomas*, Jan L. Ruben, Jaap J. ten Bosch, Marie-Charlotte D. N. J. M. Huysmans

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    29 Citations (Scopus)

    Abstract

    For in situ studies into caries prevention, sterilization of tooth samples is essential. However, sterilization may influence the caries process itself. The aim of this study was to assess the effect of sterilising sound human enamel and dentin with ethylene oxide on lesion depth and mineral loss before and after in vitro demineralization. Lesion depth and mineral loss were measured using transversal microradiography (TMR). The experiment was carried out with 32 enamel and 32 dentin samples. We found a significant reduction of lesion depth due to sterilization in demineralized enamel (-9.8 mu m; 95% CI: -15.1 to -4.4 mu m). The small effect of sterilization on demineralized enamel is considered to be irrelevant for in situ studies of de- and remineralization. (C) 2007 Elsevier Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)547-551
    Number of pages5
    JournalJOURNAL OF DENTISTRY
    Volume35
    Issue number7
    DOIs
    Publication statusPublished - Jul-2007

    Keywords

    • ethylene-oxide
    • sterilization
    • in situ
    • demineralization
    • TMR
    • INTRAORAL CARIOGENICITY TESTS
    • IRRADIATION
    • LESIONS
    • CARIES
    • TEETH

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