Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune

K Scholtmeijer*, HAB Wosten, J Springer, JGH Wessels

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.

Original languageEnglish
Pages (from-to)481-483
Number of pages3
JournalApplied and environmental microbiology
Volume67
Issue number1
Publication statusPublished - Jan-2001

Keywords

  • MESSENGER-RNA
  • TRANSFORMATION
  • METHYLATION
  • HYDROPHOBIN
  • FUNGI
  • DNA

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