TY - JOUR
T1 - Effect of sulfate concentration on glycosaminoglycan synthesis in explant cultures of bovine articular cartilage
AU - BRAND, HS
AU - VANKAMPEN, GPJ
AU - VANDESTADT, RJ
AU - Kuijer, R.
AU - VANDERKORST, JK
PY - 1989/2
Y1 - 1989/2
N2 - The effects of the sulfate- and FCS concentration on the rate of synthesis and the biochemical properties of glycosaminoglycans, synthesized in bovine articular cartilage in vitro, were studied. 20 % FCS in the culture medium stimulated the rate of synthesis. In media without FCS, the rate of synthesis decends from day 0 on. The differences in incorporation rates of [35S]-sodium sulfate and 1,6-[3H]-glucosamine-HCl into glycosaminoglycans in serum free media containing 9 μM and 900 μM sulfate were used to discuss the inorganic sulfate concentration in cartilage. In 9 μM sulfate medium, the newly synthesized glycosaminoglycans contain higher levels of unsulfated disaccharides than the endogenous glycosaminoglycans. In each culture medium, the ratio 6-sulfated disaccharides to 4-sulfated disaccharides of the newly synthesized glycosaminoglycans becomes higher after 3 days in culture. The glycosaminoglycan synthesis is underestimated, when chondrocytes are cultured in media containing less than 200 μM sulfate.
AB - The effects of the sulfate- and FCS concentration on the rate of synthesis and the biochemical properties of glycosaminoglycans, synthesized in bovine articular cartilage in vitro, were studied. 20 % FCS in the culture medium stimulated the rate of synthesis. In media without FCS, the rate of synthesis decends from day 0 on. The differences in incorporation rates of [35S]-sodium sulfate and 1,6-[3H]-glucosamine-HCl into glycosaminoglycans in serum free media containing 9 μM and 900 μM sulfate were used to discuss the inorganic sulfate concentration in cartilage. In 9 μM sulfate medium, the newly synthesized glycosaminoglycans contain higher levels of unsulfated disaccharides than the endogenous glycosaminoglycans. In each culture medium, the ratio 6-sulfated disaccharides to 4-sulfated disaccharides of the newly synthesized glycosaminoglycans becomes higher after 3 days in culture. The glycosaminoglycan synthesis is underestimated, when chondrocytes are cultured in media containing less than 200 μM sulfate.
U2 - 10.1016/0309-1651(89)90061-1
DO - 10.1016/0309-1651(89)90061-1
M3 - Article
C2 - 2720791
SN - 0309-1651
VL - 13
SP - 153
EP - 162
JO - Cell Biology International Reports
JF - Cell Biology International Reports
IS - 2
ER -