Effect of the alginate composition on the biocompatibility of alginate-polylysine microcapsules

P De Vos*, B De Haan, R Van Schilfgaarde

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    229 Citations (Scopus)

    Abstract

    Alginate-polylysine (PLL) capsules are commonly applied for immunoprotection of endocrine tissues. Alginate is composed of mannuronic acid (M) and guluronic acid (G). Different types of alginate have different ratios of G to M, but little is known of the influence of these differences on biocompatibility. Therefore, we have investigated in vivo the effect of the G-content of the alginate on the biocompatibility of the capsules. Capsules prepared of commercially available alginates with either a high or an intermediate G-content were implanted in the peritoneal cavity of rats and retrieved one month later for histological evaluation. The fibrotic reaction was more severe against high-G alginate capsules than to intermediate-G alginate capsules. The majority of the high-G capsules proved to be overgrown and adherent to the abdominal organs whereas with intermediate-G alginate most capsules were found freely floating in the peritoneal cavity and free of any adhesion of cells. This was not caused by the alginate as such but rather by inadequate binding of high-G alginate to PLL since in the absence of PLL, i.e. with beads instead of capsules, no fibrotic reaction was observed. As high-G alginates have beneficial effects for islet encapsulation, efforts should be made to apply polycations which more effectively interact with high-G alginate than PLL. (C) 1997 Elsevier Science Limited.

    Original languageEnglish
    Pages (from-to)273-278
    Number of pages6
    JournalBiomaterials
    Volume18
    Issue number3
    DOIs
    Publication statusPublished - Feb-1997

    Keywords

    • microencapsulation
    • alginate
    • poly-L-lysine
    • biocompatibility
    • peritoneal cavity
    • BIOARTIFICIAL PANCREAS
    • ISLETS
    • MICROENCAPSULATION
    • MEMBRANES
    • CAPSULES
    • RATS
    • TRANSPLANTATION
    • POLYSACCHARIDES
    • ENCAPSULATION
    • PERMEABILITY

    Cite this