TY - JOUR
T1 - Effects of Drugs Interfering with Dopamine and Noradrenaline Biosynthesis on the Endogenous 3,4‐ Dihydroxyphenylalanine Levels in Rat Brain
AU - Westerink, B. H. C.
AU - van Es, T. P.
AU - Spaan, S. J.
PY - 1982
Y1 - 1982
N2 - A chemical assay of 3,4‐dihydroxyphenylalanine (DOPA) in nervous tissue is described. The method is based on a rapidly performed isolation of DOPA on small Sephadex G‐10 columns, followed by reverse‐phase HPLC with a trichloroacetic acid‐containing eluent, in conjunction with a rotating disk electrochemical detector. The detection limit of the assay (about 100 pg/tissue sample) permits a detailed investigation of the regional distribution of endogenous DOPA levels in the rat brain. DOPA as well as dopamine (DA) could be quantified in the same chromatographic run. The assay was applied to a study of the effects of α‐methyl‐p‐tyrosine, apomorphine, chlorpromazine, clonidine, γ‐butyrolactone, haloperidol, morphine, oxotremorine, pargyline, reserpine, and tyrosine methylester on the concentration of DOPA in the striatum, hypothalamus, frontal cortex, and cerebellum of the rat brain. Drugs known to interact with DA biosynthesis all caused characteristic changes of the DOPA content in the striatum and not in nondopaminergic brain areas. A close correlation existed between drug‐induced changes in tyrosine hydroxylase activity and changes in the DOPA content in the striatum. Tyrosine methylester increased DOPA concentrations in all brain areas studied.
AB - A chemical assay of 3,4‐dihydroxyphenylalanine (DOPA) in nervous tissue is described. The method is based on a rapidly performed isolation of DOPA on small Sephadex G‐10 columns, followed by reverse‐phase HPLC with a trichloroacetic acid‐containing eluent, in conjunction with a rotating disk electrochemical detector. The detection limit of the assay (about 100 pg/tissue sample) permits a detailed investigation of the regional distribution of endogenous DOPA levels in the rat brain. DOPA as well as dopamine (DA) could be quantified in the same chromatographic run. The assay was applied to a study of the effects of α‐methyl‐p‐tyrosine, apomorphine, chlorpromazine, clonidine, γ‐butyrolactone, haloperidol, morphine, oxotremorine, pargyline, reserpine, and tyrosine methylester on the concentration of DOPA in the striatum, hypothalamus, frontal cortex, and cerebellum of the rat brain. Drugs known to interact with DA biosynthesis all caused characteristic changes of the DOPA content in the striatum and not in nondopaminergic brain areas. A close correlation existed between drug‐induced changes in tyrosine hydroxylase activity and changes in the DOPA content in the striatum. Tyrosine methylester increased DOPA concentrations in all brain areas studied.
U2 - 10.1111/j.1471-4159.1982.tb04699.x
DO - 10.1111/j.1471-4159.1982.tb04699.x
M3 - Article
SN - 0022-3042
VL - 39
SP - 44
EP - 51
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 1
ER -