Efficient Enzymatic Cyclization of Disulfide-rich Peptides using Peptide Ligases

Marcel Schmidt, Yen-Hua Huang, Eduardo F Texeira de Oliveira, Ana Toplak, Hein J Wijma, Dick B Janssen, Jan H van Maarseveen, David J Craik, Timo Nuijens*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Disulfide-rich macrocyclic peptides, e.g. cyclotides, represent a promising class of molecules with potential therapeutic use. Despite their potential their efficient synthesis at large scale still represents a major challenge. Here we report new chemo-enzymatic strategies using peptiligase variants, i.a. omniligase-1, for the efficient and scalable one-pot cyclization and folding of the native cyclotides MCoTI-II, kalata B1 and variants thereof as well as of the θ-defensin RTD-1. The synthesis of the kalata B1 variant T20K was successfully demonstrated at multi-gram scale. Several ligation sites for each macrocycle render this approach highly flexible and facilitate both the larger scale manufacture and the engineering of bioactive, grafted cyclotide variants, therefore clearly offering a valuable and powerful extension of the existing toolbox of enzymes for peptide head-to-tail cyclization.

Original languageEnglish
Pages (from-to)1524-1529
Number of pages6
JournalChemBioChem
Volume20
Issue number12
Early online date8-Feb-2019
DOIs
Publication statusPublished - 14-Jun-2019

Keywords

  • RECOMBINANT EXPRESSION
  • BACKBONE CYCLIZATION
  • MACROCYCLIC PEPTIDES
  • CIRCULAR PROTEINS
  • MEDIATED LIGATION
  • CYCLOTIDES
  • BIOSYNTHESIS
  • TEMPLATES
  • ESTERS
  • RTD-1

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