Abstract
Polarization transfer between spin-1/2 nuclei and quadrupolar spin-1 nuclei such as
N in solid-state NMR is severely challenged by the typical presence of large quadrupole coupling interactions. This has effectively prevented the use of the abundant
N spin as a probe to structural information and its use as an element in multi-dimensional solid-state NMR correlation experiments for assignment and structural characterization. In turn, this has been a contributing factor to the extensive use of isotope labeling in biological solid-state NMR, where
N is replaced with
N. The alternative strategy of using the abundant
N spins calls for methods enabling efficient polarization transfer between
N and its binding partners. This work demonstrates that the recently introduced
CP transfer method can be optimized to achieve efficient
H
N polarization transfer under magic angle spinning conditions. The method is demonstrated numerically and experimentally on powder samples of NH4NO3 and l-alanine.
N in solid-state NMR is severely challenged by the typical presence of large quadrupole coupling interactions. This has effectively prevented the use of the abundant
N spin as a probe to structural information and its use as an element in multi-dimensional solid-state NMR correlation experiments for assignment and structural characterization. In turn, this has been a contributing factor to the extensive use of isotope labeling in biological solid-state NMR, where
N is replaced with
N. The alternative strategy of using the abundant
N spins calls for methods enabling efficient polarization transfer between
N and its binding partners. This work demonstrates that the recently introduced
CP transfer method can be optimized to achieve efficient
H
N polarization transfer under magic angle spinning conditions. The method is demonstrated numerically and experimentally on powder samples of NH4NO3 and l-alanine.
Original language | English |
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Pages (from-to) | 85-89 |
Number of pages | 5 |
Journal | Journal of Magnetic Resonance |
Volume | 244 |
DOIs | |
Publication status | Published - 2014 |
Externally published | Yes |