Elastic moduli of polyelectrolyte multilayer films regulate endothelium-blood interaction under dynamic conditions

Gabriela Imbir*, Klaudia Trembecka-Wójciga, Piotr Ozga, Romana Schirhagl, Aldona Mzyk

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
35 Downloads (Pure)

Abstract

A broad spectrum of biomaterials has been explored in order to design cardiovascular implants of sufficient hemocompatibility. Most of them were extensively tested for the ability to facilitate repopulation by patient cells. It was shown that stiffness, surface roughness, or hydrophilicity of polyelectrolyte films have an impact on adhesion, proliferation, and differentiation of cells. At the same time, it is still unknown how these properties influence cell functionality and as a consequence interactions with blood components under dynamic conditions. In this study, we aimed to determine the impact of chemical cross-linking of Chitosan (Chi) and Chrondroitin Sulphate (CS) on endothelium-blood cross-talk. We have found that the morphology of the endothelium monolayer was not altered by changes in coating properties. However, free radical generation by endothelial cells varied depending on the elastic properties of the coating. Simultaneously, we have observed a significant decrease in the level of adhering and circulating active platelets as well as aggregates when the endothelium monolayer was formed on stiffer films than on the other coating variants. Moreover, the same type of films has promoted significantly higher adhesion of blood morphotic elements when they were not functionalized by endothelium. The observed changes in hemocompatibility indicate the importance of a design of coatings that will promote cellularization in vivo in a relatively short time and which will regulate cell function.

Original languageEnglish
Article number113269
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume225
DOIs
Publication statusPublished - May-2023

Keywords

  • Blood-material interaction
  • Endothelial cells
  • Nanodiamond magnetometry
  • Polyelectrolyte multilayer
  • T1 relaxometry

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