Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes

Pauline Lefrançois; Fanny Girard-Sahun; Vasilica Badets; Franck Clément; Stéphane Arbault

doi:10.1002/elan.202060456

Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes

Pauline Lefrançois
, Fanny Girard-Sahun
, Vasilica Badets
, Franck Clément
, Stéphane Arbault^*

^*Corresponding author for this work

Enzymology

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

60 Downloads (Pure)

Abstract

The reactivity of platinized ultramicroelectrodes (Pt-black UMEs) towards superoxide anion O₂^.−, an unstable Reactive Oxygen Species (ROS), and its relatives, H₂O₂ and O₂, was studied. Voltammetric studies in PBS demonstrate that Pt-black UMEs provide: i) a well-resolved reversible redox signature for O₂^.− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H₂O₂ at pH 7.4. The oxygen reduction reaction (ORR) at Pt-black surfaces solely yields H₂O₂ (2 electrons/2 H⁺) at physiological pH. Consequently, Pt-black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico-chemical investigations.

Original language	English
Pages (from-to)	882-890
Number of pages	9
Journal	Electroanalysis
Volume	33
Issue number	4
DOIs	https://doi.org/10.1002/elan.202060456
Publication status	Published - Apr-2021

Keywords

cold atmospheric plasma
hydrogen peroxide
reactive oxygen species
superoxide
ultramicroelectrode

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Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized MicroelectrodesFinal publisher's version, 4.04 MBLicence: Taverne

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@article{325e01930c474e7faad3450cebabaab8,

title = "Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes",

abstract = "The reactivity of platinized ultramicroelectrodes (Pt-black UMEs) towards superoxide anion O2.−, an unstable Reactive Oxygen Species (ROS), and its relatives, H2O2 and O2, was studied. Voltammetric studies in PBS demonstrate that Pt-black UMEs provide: i) a well-resolved reversible redox signature for O2.− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H2O2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt-black surfaces solely yields H2O2 (2 electrons/2 H+) at physiological pH. Consequently, Pt-black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico-chemical investigations.",

keywords = "cold atmospheric plasma, hydrogen peroxide, reactive oxygen species, superoxide, ultramicroelectrode",

author = "Pauline Lefran{\c c}ois and Fanny Girard-Sahun and Vasilica Badets and Franck Cl{\'e}ment and St{\'e}phane Arbault",

note = "Funding Information: This work was financially supported by the Centre National de la Recherche Scientifique (CNRS), the French Ministry of Research (MESR) and the Agence Nationale de la Recherche (ANR, PLASMAREGEN project, no. ANR‐14‐CE16‐0007‐01). Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",

year = "2021",

month = apr,

doi = "10.1002/elan.202060456",

language = "English",

volume = "33",

pages = "882--890",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley",

number = "4",

}

TY - JOUR

T1 - Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes

AU - Lefrançois, Pauline

AU - Girard-Sahun, Fanny

AU - Badets, Vasilica

AU - Clément, Franck

AU - Arbault, Stéphane

N1 - Funding Information: This work was financially supported by the Centre National de la Recherche Scientifique (CNRS), the French Ministry of Research (MESR) and the Agence Nationale de la Recherche (ANR, PLASMAREGEN project, no. ANR‐14‐CE16‐0007‐01). Publisher Copyright: © 2020 Wiley-VCH GmbH

PY - 2021/4

Y1 - 2021/4

N2 - The reactivity of platinized ultramicroelectrodes (Pt-black UMEs) towards superoxide anion O2.−, an unstable Reactive Oxygen Species (ROS), and its relatives, H2O2 and O2, was studied. Voltammetric studies in PBS demonstrate that Pt-black UMEs provide: i) a well-resolved reversible redox signature for O2.− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H2O2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt-black surfaces solely yields H2O2 (2 electrons/2 H+) at physiological pH. Consequently, Pt-black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico-chemical investigations.

AB - The reactivity of platinized ultramicroelectrodes (Pt-black UMEs) towards superoxide anion O2.−, an unstable Reactive Oxygen Species (ROS), and its relatives, H2O2 and O2, was studied. Voltammetric studies in PBS demonstrate that Pt-black UMEs provide: i) a well-resolved reversible redox signature for O2.− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H2O2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt-black surfaces solely yields H2O2 (2 electrons/2 H+) at physiological pH. Consequently, Pt-black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico-chemical investigations.

KW - cold atmospheric plasma

KW - hydrogen peroxide

KW - reactive oxygen species

KW - superoxide

KW - ultramicroelectrode

UR - https://www.scopus.com/pages/publications/85097014587

U2 - 10.1002/elan.202060456

DO - 10.1002/elan.202060456

M3 - Article

AN - SCOPUS:85097014587

SN - 1040-0397

VL - 33

SP - 882

EP - 890

JO - Electroanalysis

JF - Electroanalysis

IS - 4

ER -

Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes

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