Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome

Marco Bucci; Anna C Karmi; Patricia Iozzo; Barbara A Fielding; Antti Viljanen; Robert M Badeau; Ronald Borra; Virva Saunavaara; Tam Pham; Jarna C Hannukainen; Kari Kalliokoski; Merja Haaparanta-Solin; Tapio Viljanen; Riitta Parkkola; Keith N Frayn; Pirjo Nuutila

doi:10.1007/s00125-014-3402-x

Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome

Marco Bucci, Anna C Karmi, Patricia Iozzo, Barbara A Fielding, Antti Viljanen, Robert M Badeau, Ronald Borra, Virva Saunavaara, Tam Pham, Jarna C Hannukainen, Kari Kalliokoski, Merja Haaparanta-Solin, Tapio Viljanen, Riitta Parkkola, Keith N Frayn, Pirjo Nuutila

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

AIMS/HYPOTHESIS: Obesity causes an imbalance in fat mass distribution between visceral and subcutaneous adipose tissue (AT) depots. We tested the hypothesis that this relates to increased NEFA uptake between these depots in obese compared with healthy participants. Second, we hypothesised that a diet very low in energy (very low calorie diet [VLCD]) decreases fat mass in obese participants and that this is associated with the decline in NEFA uptake.

METHODS: NEFA uptake in AT depots was measured with [(18)F]-fluoro-6-thia-heptadecanoic acid ((18)F-FTHA) and positron emission tomography (PET) in 18 obese participants with the metabolic syndrome before and after a 6 week VLCD. Whole body fat oxidation was measured using indirect calorimetry and [U-(13)C]palmitate. Sixteen non-obese participants were controls.

RESULTS: Obese participants had >100% higher (p < 0.0001) NEFA uptake in the visceral and subcutaneous abdominal AT depots than controls. VLCD decreased AT mass in all regions (12% to 21%), but NEFA uptake was decreased significantly (18%; p < 0.006) only in the femoral AT. Whole body carbohydrate oxidation decreased, while fat oxidation increased.

CONCLUSIONS/INTERPRETATION: The data demonstrate that weight loss caused by VLCD does not affect abdominal fasting NEFA uptake rates. We found that visceral fat takes up more NEFAs than subcutaneous AT depots, even after weight loss.

Original language	English
Pages (from-to)	158-164
Number of pages	7
Journal	Diabetologia
Volume	58
Issue number	1
DOIs	https://doi.org/10.1007/s00125-014-3402-x
Publication status	Published - Jan-2015
Externally published	Yes

Keywords

Adult
Caloric Restriction
Calorimetry, Indirect
Fatty Acids, Nonesterified
Female
Humans
Intra-Abdominal Fat
Male
Metabolic Syndrome
Middle Aged
Muscle, Skeletal
Obesity
Positron-Emission Tomography
Radiography
Subcutaneous Fat
Weight Loss
Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

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Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome
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Bucci, M., Karmi, A. C., Iozzo, P., Fielding, B. A., Viljanen, A., Badeau, R. M., Borra, R., Saunavaara, V., Pham, T., Hannukainen, J. C., Kalliokoski, K., Haaparanta-Solin, M., Viljanen, T., Parkkola, R., Frayn, K. N., & Nuutila, P. (2015). Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome. Diabetologia, 58(1), 158-164. https://doi.org/10.1007/s00125-014-3402-x

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title = "Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome",

abstract = "AIMS/HYPOTHESIS: Obesity causes an imbalance in fat mass distribution between visceral and subcutaneous adipose tissue (AT) depots. We tested the hypothesis that this relates to increased NEFA uptake between these depots in obese compared with healthy participants. Second, we hypothesised that a diet very low in energy (very low calorie diet [VLCD]) decreases fat mass in obese participants and that this is associated with the decline in NEFA uptake.METHODS: NEFA uptake in AT depots was measured with [(18)F]-fluoro-6-thia-heptadecanoic acid ((18)F-FTHA) and positron emission tomography (PET) in 18 obese participants with the metabolic syndrome before and after a 6 week VLCD. Whole body fat oxidation was measured using indirect calorimetry and [U-(13)C]palmitate. Sixteen non-obese participants were controls.RESULTS: Obese participants had >100% higher (p < 0.0001) NEFA uptake in the visceral and subcutaneous abdominal AT depots than controls. VLCD decreased AT mass in all regions (12% to 21%), but NEFA uptake was decreased significantly (18%; p < 0.006) only in the femoral AT. Whole body carbohydrate oxidation decreased, while fat oxidation increased.CONCLUSIONS/INTERPRETATION: The data demonstrate that weight loss caused by VLCD does not affect abdominal fasting NEFA uptake rates. We found that visceral fat takes up more NEFAs than subcutaneous AT depots, even after weight loss.",

keywords = "Adult, Caloric Restriction, Calorimetry, Indirect, Fatty Acids, Nonesterified, Female, Humans, Intra-Abdominal Fat, Male, Metabolic Syndrome, Middle Aged, Muscle, Skeletal, Obesity, Positron-Emission Tomography, Radiography, Subcutaneous Fat, Weight Loss, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't",

author = "Marco Bucci and Karmi, {Anna C} and Patricia Iozzo and Fielding, {Barbara A} and Antti Viljanen and Badeau, {Robert M} and Ronald Borra and Virva Saunavaara and Tam Pham and Hannukainen, {Jarna C} and Kari Kalliokoski and Merja Haaparanta-Solin and Tapio Viljanen and Riitta Parkkola and Frayn, {Keith N} and Pirjo Nuutila",

year = "2015",

month = jan,

doi = "10.1007/s00125-014-3402-x",

language = "English",

volume = "58",

pages = "158--164",

journal = "Diabetologia",

issn = "0012-186X",

publisher = "Springer",

number = "1",

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Bucci, M, Karmi, AC, Iozzo, P, Fielding, BA, Viljanen, A, Badeau, RM, Borra, R, Saunavaara, V, Pham, T, Hannukainen, JC, Kalliokoski, K, Haaparanta-Solin, M, Viljanen, T, Parkkola, R, Frayn, KN & Nuutila, P 2015, 'Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome', Diabetologia, vol. 58, no. 1, pp. 158-164. https://doi.org/10.1007/s00125-014-3402-x

TY - JOUR

T1 - Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome

AU - Bucci, Marco

AU - Karmi, Anna C

AU - Iozzo, Patricia

AU - Fielding, Barbara A

AU - Viljanen, Antti

AU - Badeau, Robert M

AU - Borra, Ronald

AU - Saunavaara, Virva

AU - Pham, Tam

AU - Hannukainen, Jarna C

AU - Kalliokoski, Kari

AU - Haaparanta-Solin, Merja

AU - Viljanen, Tapio

AU - Parkkola, Riitta

AU - Frayn, Keith N

AU - Nuutila, Pirjo

PY - 2015/1

Y1 - 2015/1

N2 - AIMS/HYPOTHESIS: Obesity causes an imbalance in fat mass distribution between visceral and subcutaneous adipose tissue (AT) depots. We tested the hypothesis that this relates to increased NEFA uptake between these depots in obese compared with healthy participants. Second, we hypothesised that a diet very low in energy (very low calorie diet [VLCD]) decreases fat mass in obese participants and that this is associated with the decline in NEFA uptake.METHODS: NEFA uptake in AT depots was measured with [(18)F]-fluoro-6-thia-heptadecanoic acid ((18)F-FTHA) and positron emission tomography (PET) in 18 obese participants with the metabolic syndrome before and after a 6 week VLCD. Whole body fat oxidation was measured using indirect calorimetry and [U-(13)C]palmitate. Sixteen non-obese participants were controls.RESULTS: Obese participants had >100% higher (p < 0.0001) NEFA uptake in the visceral and subcutaneous abdominal AT depots than controls. VLCD decreased AT mass in all regions (12% to 21%), but NEFA uptake was decreased significantly (18%; p < 0.006) only in the femoral AT. Whole body carbohydrate oxidation decreased, while fat oxidation increased.CONCLUSIONS/INTERPRETATION: The data demonstrate that weight loss caused by VLCD does not affect abdominal fasting NEFA uptake rates. We found that visceral fat takes up more NEFAs than subcutaneous AT depots, even after weight loss.

AB - AIMS/HYPOTHESIS: Obesity causes an imbalance in fat mass distribution between visceral and subcutaneous adipose tissue (AT) depots. We tested the hypothesis that this relates to increased NEFA uptake between these depots in obese compared with healthy participants. Second, we hypothesised that a diet very low in energy (very low calorie diet [VLCD]) decreases fat mass in obese participants and that this is associated with the decline in NEFA uptake.METHODS: NEFA uptake in AT depots was measured with [(18)F]-fluoro-6-thia-heptadecanoic acid ((18)F-FTHA) and positron emission tomography (PET) in 18 obese participants with the metabolic syndrome before and after a 6 week VLCD. Whole body fat oxidation was measured using indirect calorimetry and [U-(13)C]palmitate. Sixteen non-obese participants were controls.RESULTS: Obese participants had >100% higher (p < 0.0001) NEFA uptake in the visceral and subcutaneous abdominal AT depots than controls. VLCD decreased AT mass in all regions (12% to 21%), but NEFA uptake was decreased significantly (18%; p < 0.006) only in the femoral AT. Whole body carbohydrate oxidation decreased, while fat oxidation increased.CONCLUSIONS/INTERPRETATION: The data demonstrate that weight loss caused by VLCD does not affect abdominal fasting NEFA uptake rates. We found that visceral fat takes up more NEFAs than subcutaneous AT depots, even after weight loss.

KW - Adult

KW - Caloric Restriction

KW - Calorimetry, Indirect

KW - Fatty Acids, Nonesterified

KW - Female

KW - Humans

KW - Intra-Abdominal Fat

KW - Male

KW - Metabolic Syndrome

KW - Middle Aged

KW - Muscle, Skeletal

KW - Obesity

KW - Positron-Emission Tomography

KW - Radiography

KW - Subcutaneous Fat

KW - Weight Loss

KW - Controlled Clinical Trial

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00125-014-3402-x

DO - 10.1007/s00125-014-3402-x

M3 - Article

C2 - 25331375

SN - 0012-186X

VL - 58

SP - 158

EP - 164

JO - Diabetologia

JF - Diabetologia

IS - 1

ER -

Enhanced fatty acid uptake in visceral adipose tissue is not reversed by weight loss in obese individuals with the metabolic syndrome

Abstract

Keywords

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