Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode

M. M. Azimatun Nur*, G. M. Garcia, P. Boelen, A. G. J. Buma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)
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Abstract

Palm oil mill effluent (POME) is well known as agricultural wastewater that has a high potential as a medium for microalgal growth due to its high macro- and micronutrient content. The cyanobacterium Arthrospira platensis is considered as a species with a high C-phycocyanin (C-PC) content which is important for fine chemical and pharmaceutical applications. However, cultivation of A. platensis on POME to produce economically feasible amounts of C-PC has not been well explored. For this, environmental, nutritional, and cultivation modes (batch, semi-continuous) were varied to optimize C-PC productivity when cultivated at various POME concentrations. Arthrospira platensis was found to grow well on POME. Highest biomass and C-PC concentrations were found on 30–100% POME. Central composite rotatable design (CCRD) response surface methodology demonstrated that C-PC productivity was influenced by urea addition at the optimum salinity. The highest C-PC productivity was found on 100% POME during semi-continuous cultivation, while the addition of phosphorus and urea did not significantly improve C-PC productivity. By applying semi-continuous cultivation with 50% POME at the first stage and 100% POME at the second stage, a similarly high C-PC productivity (4.08 ± 1.3 mg L−1 day−1) was achieved as compared with (artificial) Zarrouk medium during batch cultivation. We conclude that, when using a two-stage semi-continuous cultivation process, A. platensis can produce economically feasible amounts of C-PC when cultivated on 100% POME.
Original languageEnglish
Pages (from-to)2855-2867
Number of pages13
JournalJournal of Applied Phycology
Volume31
Issue number5
Early online date8-May-2019
DOIs
Publication statusPublished - Oct-2019

Keywords

  • Wastewater
  • Light intensity
  • Urea
  • C-PC
  • Arthrospira platensis
  • semi-continuous
  • SPIRULINA-PLATENSIS
  • LIPID PRODUCTION
  • WASTE-WATER
  • BIOMASS
  • MICROALGAE
  • OPTIMIZATION
  • EXTRACTION
  • GROWTH
  • ANTIOXIDANT
  • REMOVAL

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