Enhancement of the Immunogenicity and Protective Efficacy of a Mucosal Influenza Subunit Vaccine by the Saponin Adjuvant GPI-0100

Heng Liu, Harshad P. Patil, Jacqueline de Vries-Idema, Jan Wilschut, Anke Huckriede*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)
228 Downloads (Pure)

Abstract

Identification of safe and effective adjuvants remains an urgent need for the development of inactivated influenza vaccines for mucosal administration. Here, we used a murine challenge model to evaluate the adjuvant activity of GPI-0100, a saponin-derived adjuvant, on influenza subunit vaccine administered via the intranasal or the intrapulmonary route. Balb/c mice were immunized with 1 mu g A/PR/8 (H1N1) subunit antigen alone or in combination with varying doses of GPI-0100. The addition of GPI-0100 was required for induction of mucosal and systemic antibody responses to intranasally administered influenza vaccine and significantly enhanced the immunogenicity of vaccine administered via the intrapulmonary route. Remarkably, GPI-0100-adjuvanted influenza vaccine given at a low dose of 2x1 mu g either in the nares or directly into the lungs provided complete protection against homologous influenza virus infection.

Original languageEnglish
Article numbere52135
Number of pages10
JournalPLoS ONE
Volume7
Issue number12
DOIs
Publication statusPublished - 17-Dec-2012

Keywords

  • RESPIRATORY SYNCYTIAL VIRUS
  • PULMONARY DELIVERY
  • BELLS-PALSY
  • IMMUNITY
  • INFECTION
  • RESPONSES
  • ANTIBODY
  • PROTEIN
  • CELLS
  • TRACT

Cite this