ENZYME KINETICS FOR SYSTEMS BIOLOGY: WHEN, WHY AND HOW

Malgorzata Adamczyk*, Karen van Eunen, Barbara M. Bakker, Hans V. Westerhoff

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

12 Citations (Scopus)

Abstract

In vitro enzymatic assays of cell-free extracts offer an opportunity to assess in vivo enzyme concentrations. If performed under conditions that resemble the conditions in vivo, they may also reveal some of the capacities and properties of the same enzymes in vivo; we shall call this the ex vivo approach. The kinetic characterization of purified enzymes has yet a different utility for systems biology, as does the in vivo determination of enzyme activities. All these approaches are different, and it is becoming important that the appropriate approach be used for the intended purpose. Here, we therefore discuss five approaches to the measurement of enzyme activity in terms of the source of the enzyme activity, the identity of the assay medium, and the purpose of the assay.

Original languageEnglish
Title of host publicationMETHODS IN ENZYMOLOGY, VOL 500
EditorsD Jameson, M Verma, HV Westerhoff
Place of PublicationSAN DIEGO
PublisherAcademic Press
Pages233-257
Number of pages25
ISBN (Print)978-0-12-385118-5
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Enzymology
PublisherELSEVIER ACADEMIC PRESS INC
Volume500
ISSN (Print)0076-6879

Keywords

  • TIME-DEPENDENT REGULATION
  • CELL NMR-SPECTROSCOPY
  • SACCHAROMYCES-CEREVISIAE
  • IN-VIVO
  • METABOLIC-REGULATION
  • NITROGEN STARVATION
  • GLYCOLYTIC-ENZYMES
  • CHEMOSTAT CULTURES
  • CARBON METABOLISM
  • ESCHERICHIA-COLI

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