Epigenetic age-predictor for mice based on three CpG sites

Yang Han, Monika Eipel, Julia Franzen, Vadim Sakk, Bertien Dethmers-Ausema, Laura Yndriago, Ander Izeta, Gerald de Haan, Hartmut Geiger, Wolfgang Wagner*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)
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Abstract

Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise estimation of chronological age in murine blood samples, too. DBA/2 mice revealed accelerated epigenetic aging as compared to C57BL6 mice, which is in line with their shorter life-expectancy. The three-CpG-predictor provides a simple and cost-effective biomarker to determine biological age in large intervention studies with mice.

Original languageEnglish
Article number37462
Number of pages10
JournaleLife
Volume7
DOIs
Publication statusPublished - 24-Aug-2018

Keywords

  • DNA METHYLATION AGE
  • LIFE-SPAN
  • EXPECTANCY
  • LONGEVITY

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