Escherichia coli Phosphoenolpyruvate Dependent Phosphotransferase System: Complete Purification of Enzyme I by Hydrophobic Interaction Chromatography

G.T. Robillard, G. Dooijewaard, J. Lolkema

Research output: Contribution to journalArticleAcademic

50 Citations (Scopus)

Abstract

We have observed that EI possesses extremely hydrophobic surface regions. In light of this property, a high yield, rapid procedure has been developed for the complete purification of EI using solely hydrophobic interaction chromatography on commercially available resins. The entire procedure can be completed in 4-5 days with a 55-65% recovery of EI activity and an 870-fold purification.
Original languageEnglish
Pages (from-to)2984-2989
Number of pages6
JournalBiochemistry
Volume18
Issue number14
DOIs
Publication statusPublished - 1979

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