Escherichia coli Phosphoenolpyruvate Dependent Phosphotransferase System: Complete Purification of Enzyme I by Hydrophobic Interaction Chromatography

G.T. Robillard; G. Dooijewaard; J. Lolkema

doi:10.1021/bi00581a012

Escherichia coli Phosphoenolpyruvate Dependent Phosphotransferase System: Complete Purification of Enzyme I by Hydrophobic Interaction Chromatography

G.T. Robillard, G. Dooijewaard, J. Lolkema

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic

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Abstract

We have observed that EI possesses extremely hydrophobic surface regions. In light of this property, a high yield, rapid procedure has been developed for the complete purification of EI using solely hydrophobic interaction chromatography on commercially available resins. The entire procedure can be completed in 4-5 days with a 55-65% recovery of EI activity and an 870-fold purification.

Original language	English
Pages (from-to)	2984-2989
Number of pages	6
Journal	Biochemistry
Volume	18
Issue number	14
DOIs	https://doi.org/10.1021/bi00581a012
Publication status	Published - 1979

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title = "Escherichia coli Phosphoenolpyruvate Dependent Phosphotransferase System: Complete Purification of Enzyme I by Hydrophobic Interaction Chromatography",

abstract = "We have observed that EI possesses extremely hydrophobic surface regions. In light of this property, a high yield, rapid procedure has been developed for the complete purification of EI using solely hydrophobic interaction chromatography on commercially available resins. The entire procedure can be completed in 4-5 days with a 55-65% recovery of EI activity and an 870-fold purification.",

author = "G.T. Robillard and G. Dooijewaard and J. Lolkema",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen. Groningen Biomolecular Sciences and Biotechnology Institute",

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T2 - Complete Purification of Enzyme I by Hydrophobic Interaction Chromatography

AU - Robillard, G.T.

AU - Dooijewaard, G.

AU - Lolkema, J.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen. Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1979

Y1 - 1979

N2 - We have observed that EI possesses extremely hydrophobic surface regions. In light of this property, a high yield, rapid procedure has been developed for the complete purification of EI using solely hydrophobic interaction chromatography on commercially available resins. The entire procedure can be completed in 4-5 days with a 55-65% recovery of EI activity and an 870-fold purification.

AB - We have observed that EI possesses extremely hydrophobic surface regions. In light of this property, a high yield, rapid procedure has been developed for the complete purification of EI using solely hydrophobic interaction chromatography on commercially available resins. The entire procedure can be completed in 4-5 days with a 55-65% recovery of EI activity and an 870-fold purification.

U2 - 10.1021/bi00581a012

DO - 10.1021/bi00581a012

M3 - Article

VL - 18

SP - 2984

EP - 2989

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

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