Estimation of individual ultraviolet spectra in incomplete two-component separations by high-performance liquid chromatography

J. H. van Tongeren; J. W. Weyland; H. van der Voet; P. M. J. Coenegracht

doi:10.1016/S0003-2670(00)81748-1

Estimation of individual ultraviolet spectra in incomplete two-component separations by high-performance liquid chromatography

J. H. van Tongeren, J. W. Weyland, H. van der Voet, P. M. J. Coenegracht

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Abstract

A method is described for the estimation of spectral features in a two-component chromatographic peak by means of a u.v. diode-array detector. The calculation relies on the assumption that the front of a fused chromatographic peak contains a single pure component. The spectrum of this component is used in calculating the concentration profile of the other component, thus allowing the determination of a solution band for the spectrum of the second component. The boundaries of the solution band are based on non-negativity restrictions of chromatographic and spectral features. The method does not require the use of principal components analysis.

Original language	English
Pages (from-to)	245-253
Number of pages	9
Journal	Analytica Chimica Acta
Volume	170
Issue number	APR
DOIs	https://doi.org/10.1016/S0003-2670(00)81748-1
Publication status	Published - 1985

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Estimation of individual ultraviolet spectra in incomplete two-component separations by high-performance liquid chromatography
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abstract = "A method is described for the estimation of spectral features in a two-component chromatographic peak by means of a u.v. diode-array detector. The calculation relies on the assumption that the front of a fused chromatographic peak contains a single pure component. The spectrum of this component is used in calculating the concentration profile of the other component, thus allowing the determination of a solution band for the spectrum of the second component. The boundaries of the solution band are based on non-negativity restrictions of chromatographic and spectral features. The method does not require the use of principal components analysis.",

author = "{van Tongeren}, {J. H.} and Weyland, {J. W.} and {van der Voet}, H. and Coenegracht, {P. M. J.}",

year = "1985",

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AU - van Tongeren, J. H.

AU - Weyland, J. W.

AU - van der Voet, H.

AU - Coenegracht, P. M. J.

PY - 1985

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N2 - A method is described for the estimation of spectral features in a two-component chromatographic peak by means of a u.v. diode-array detector. The calculation relies on the assumption that the front of a fused chromatographic peak contains a single pure component. The spectrum of this component is used in calculating the concentration profile of the other component, thus allowing the determination of a solution band for the spectrum of the second component. The boundaries of the solution band are based on non-negativity restrictions of chromatographic and spectral features. The method does not require the use of principal components analysis.

AB - A method is described for the estimation of spectral features in a two-component chromatographic peak by means of a u.v. diode-array detector. The calculation relies on the assumption that the front of a fused chromatographic peak contains a single pure component. The spectrum of this component is used in calculating the concentration profile of the other component, thus allowing the determination of a solution band for the spectrum of the second component. The boundaries of the solution band are based on non-negativity restrictions of chromatographic and spectral features. The method does not require the use of principal components analysis.

U2 - 10.1016/S0003-2670(00)81748-1

DO - 10.1016/S0003-2670(00)81748-1

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EP - 253

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - APR

ER -

Estimation of individual ultraviolet spectra in incomplete two-component separations by high-performance liquid chromatography

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