Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

Karsten Becker; Anders R Larsen; Robert L Skov; Gavin K Paterson; Mark A Holmes; Artur J Sabat; Alexander W Friedrich; Robin Köck; Georg Peters; André Kriegeskorte

doi:10.1128/JCM.00075-13

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

Karsten Becker^*, Anders R Larsen, Robert L Skov, Gavin K Paterson, Mark A Holmes, Artur J Sabat, Alexander W Friedrich, Robin Köck, Georg Peters, André Kriegeskorte

^*Corresponding author for this work

Microbes in Health and Disease (MHD)

Research output: Contribution to journal › Article › Academic › peer-review

23 Citations (Scopus)

Abstract

A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

Original language	English
Pages (from-to)	1917-1919
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00075-13
Publication status	Published - Jun-2013

Keywords

CASSETTE CHROMOSOME MEC
POLYMERASE-CHAIN-REACTION
RAPID DETECTION
GENE
VARIANT
IDENTIFICATION
HOMOLOG
STRAINS
GERMANY
DENMARK

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title = "Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection",

abstract = "A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.",

keywords = "CASSETTE CHROMOSOME MEC, POLYMERASE-CHAIN-REACTION, RAPID DETECTION, GENE, VARIANT, IDENTIFICATION, HOMOLOG, STRAINS, GERMANY, DENMARK",

author = "Karsten Becker and Larsen, {Anders R} and Skov, {Robert L} and Paterson, {Gavin K} and Holmes, {Mark A} and Sabat, {Artur J} and Friedrich, {Alexander W} and Robin K{\"o}ck and Georg Peters and Andr{\'e} Kriegeskorte",

year = "2013",

month = jun,

doi = "10.1128/JCM.00075-13",

language = "English",

volume = "51",

pages = "1917--1919",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "AMER SOC MICROBIOLOGY",

number = "6",

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TY - JOUR

T1 - Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

AU - Becker, Karsten

AU - Larsen, Anders R

AU - Skov, Robert L

AU - Paterson, Gavin K

AU - Holmes, Mark A

AU - Sabat, Artur J

AU - Friedrich, Alexander W

AU - Köck, Robin

AU - Peters, Georg

AU - Kriegeskorte, André

PY - 2013/6

Y1 - 2013/6

N2 - A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

AB - A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

KW - CASSETTE CHROMOSOME MEC

KW - POLYMERASE-CHAIN-REACTION

KW - RAPID DETECTION

KW - GENE

KW - VARIANT

KW - IDENTIFICATION

KW - HOMOLOG

KW - STRAINS

KW - GERMANY

KW - DENMARK

U2 - 10.1128/JCM.00075-13

DO - 10.1128/JCM.00075-13

M3 - Article

C2 - 23515551

SN - 0095-1137

VL - 51

SP - 1917

EP - 1919

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection

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