Evaluation of [C-11]-DAA1106 for imaging and quantification of neuro inflammation in a rat model of herpes encephalitis

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Abstract

Introduction: Many neurological and psychiatric disorders are associated with neuroinflammation. Positron emission tomography (PET) with [C-11]-PK11195 can be used to study neuroinflammation in these disorders. However; [C-11]-PK11195 may not be sensitive enough to visualize mild neuroinflammation. As a potentially more sensitive PET tracer for neuroinflammation, [C-11]-N-(2,5-dimethoxybenzyl)-N-(4-fluoro-2-phenoxyphenyl)-acetamide (DAA1106) was evaluated in a rat model of herpes encephalitis.

Methods: Male Wistar rats were intranasally inoculated with HSV-1 (HSE) or phosphate-buffered saline (control). At Day 6 or Day 7 after inoculation, small-animal [C-11]-DAA1106 PET scans were acquired, followed by ex vivo biodistribution. Arterial blood sampling was performed for quantification of uptake.

Results: In HSE rats, a significantly higher ex vivo, but not in vivo, uptake of [C-11]-DAA1106 was found in almost all examined brain areas (24-71%, P

Conclusions: [C-11]-DAA1106 showed a high and specific ex vivo uptake in the encephalitic rat brain. However, neuroinflammation could not be demonstrated in vivo by [C-11]-DAA1106 PET. Quantification of the uptake of [C-11]-DAA1106 using plasma sampling is not optimal, due to rapid tissue uptake, slow tissue clearance and low plasma activity. (C) 2010 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalNuclear Medicine and Biology
Volume37
Issue number1
DOIs
Publication statusPublished - Jan-2010

Keywords

  • Positron emission tomography
  • Neuroinflammation
  • Microglia
  • Herpes viruses
  • [C-11]-DAA1106
  • [C-11]-PK11195
  • TSPO
  • PERIPHERAL BENZODIAZEPINE-RECEPTOR
  • POSITRON-EMISSION-TOMOGRAPHY
  • NEUROLOGICAL DISORDERS
  • HIGH-AFFINITY
  • BINDING
  • PET
  • DAA1106
  • LIGAND
  • BRAIN

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