Evaluation of fibrosis in precision-cut tissue slices

I. M. Westra, B. T. Pham, G. M. M. Groothuis, P. Olinga*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

27 Citations (Scopus)

Abstract

1. In this review, the use of precision-cut tissue slices (PCTS) of the liver, kidney, lung and intestine in fibrosis research are evaluated and future possibilities are discussed.

2. In vivo models or techniques that are applicabless to be investigated in PCTS are discussed.

3. It is concluded that the early onset of fibrosis can be induced successfully in PCTS prepared from human and experimental animals.

4. Moreover, precision-cut slices of fibrotic tissue are effective in gaining new knowledge of the mechanisms of fibrosis and of the mode of action of potential antifibrotic drugs.

5. Both healthy and fibrotic human tissue slices will pave the way for the testing of novel therapeutic drugs to treat patients with fibrosis avoiding interspecies extrapolation.

Original languageEnglish
Pages (from-to)98-112
Number of pages15
JournalXenobiotica
Volume43
Issue number1
DOIs
Publication statusPublished - Jan-2013

Keywords

  • Precision-cut tissue slices
  • fibrosis
  • antifibrotic drugs
  • RAT-LIVER SLICES
  • IDIOPATHIC PULMONARY-FIBROSIS
  • IN-VITRO MODEL
  • INDUCED INTESTINAL FIBROSIS
  • INFLAMMATORY-BOWEL-DISEASE
  • LATE RADIATION ENTEROPATHY
  • STELLATE CELL ACTIVATION
  • INDUCED HEPATIC-FIBROSIS
  • ANTI-FIBROTIC DRUGS
  • EX-VIVO MODEL

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