Evaluation of six methylation markers derived from genome-wide screens for detection of cervical precancer and cancer

Stèfanie Dick, Lisanne Verhoef, Lise Ma De Strooper, Iuliana Ciocănea-Teodorescu, G Bea A Wisman, Chris Jlm Meijer, Maaike Cg Bleeker, Renske Dm Steenbergen, Daniëlle Am Heideman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Aim:To evaluate the triage performance of six host-cell DNA methylation markers derived from two genome-wide discovery screens for detection of cervical precancer (cervical intraepithelial neoplasia 3 [CIN]) and cancer.Materials & methods:Human papillomavirus-positive cervical scrapes of controls (0.800 after leave-one-out cross-validation. Bi-marker panelASCL1/LHX8had highest area under the curve (0.882), and detected 83.4% of CIN3 and all cervical cancers at specificity of 82.4%.Conclusion:All six methylation markers showed an equivalent, high performance for the triage of human papillomavirus-positive women using cervical scrapes with complementarity between markers.

Original languageEnglish
Pages (from-to)1569-1578
Number of pages10
JournalEpigenomics
Volume12
Issue number18
Early online date17-Sep-2020
DOIs
Publication statusPublished - Sep-2020

Keywords

  • ASCL1
  • cervical cancer
  • cervical intraepithelial neoplasia
  • cervical screening
  • DNA methylation markers
  • human genome methylation
  • human papillomavirus
  • LHX8
  • triage testing
  • HPV-POSITIVE WOMEN
  • DNA METHYLATION
  • TRIAGE
  • CYTOLOGY
  • SCRAPES
  • CADM1
  • PREVENTION
  • MANAGEMENT
  • EFFICACY

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