Ex Vivo Model in Cholestasis Research

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To mimic (human) cholestasis in vitro requires multiple triggers to establish a diseased phenotype. However, this is currently not simulated by existing in vitro models. Therefore, there is a high need for multicellular systems similar to the human physiology. In such an in vitro model, cell-cell interactions and intact bile canaliculi with functional bile flow should be present and preserved during long-term culture. Precision-cut liver slices represent an ex vivo tissue culture technique that replicates most of the multicellular characteristics of a whole liver in vivo. This chapter describes the preparation and culturing of (human) precision-cut liver slices. Furthermore, a protocol to use the precision-cut liver slices technique to predict drug-induced cholestatic liver injury is described.

Original languageEnglish
Title of host publicationExperimental Cholestasis Research
EditorsMathieu Vinken
Place of PublicationNew York
PublisherHumana Press
Number of pages12
ISBN (Electronic)978-1-4939-9420-5
ISBN (Print)978-1-4939-9419-9
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

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