ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview

Alexander Belyy; Undine Mechold; Louis Renault; Daniel Ladant

doi:10.1016/j.toxicon.2017.12.046

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview

Alexander Belyy^*, Undine Mechold, Louis Renault, Daniel Ladant

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

ExoY is one of four well-characterized Pseudomonas aeruginosa type 3 secretion system (T3SS) effectors. It is a nucleotidyl cyclase toxin that is inactive inside the bacteria, but becomes potently activated once it is delivered into the eukaryotic target cells. Recently, filamentous actin was identified as the eukaryotic cofactor that stimulates specifically ExoY enzymatic activity by several orders of magnitude. In this review, we discuss recent advances in understanding the biochemistry of nucleotidyl cyclase activity of ExoY and its regulation by interaction with filamentous actin.

Original language	English
Pages (from-to)	65-71
Number of pages	7
Journal	Toxicon
Volume	149
DOIs	https://doi.org/10.1016/j.toxicon.2017.12.046
Publication status	Published - Jul-2018
Externally published	Yes

Keywords

Actin
Bacterial protein toxin
ExoY
Pseudomonas aeruginosa
T3SS

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title = "ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview",

abstract = "ExoY is one of four well-characterized Pseudomonas aeruginosa type 3 secretion system (T3SS) effectors. It is a nucleotidyl cyclase toxin that is inactive inside the bacteria, but becomes potently activated once it is delivered into the eukaryotic target cells. Recently, filamentous actin was identified as the eukaryotic cofactor that stimulates specifically ExoY enzymatic activity by several orders of magnitude. In this review, we discuss recent advances in understanding the biochemistry of nucleotidyl cyclase activity of ExoY and its regulation by interaction with filamentous actin.",

keywords = "Actin, Bacterial protein toxin, ExoY, Pseudomonas aeruginosa, T3SS",

author = "Alexander Belyy and Undine Mechold and Louis Renault and Daniel Ladant",

note = "Funding Information: The authors thank Jenny Lee Thomassin for critical reading of the manuscript. A.B. was supported by a stipend from the Pasteur-Paris University ( PPU ) International PhD Program. The authors were funded by CNRS and by the PTR program of the Institut Pasteur ( PTR425 and PTR43-16 ). The authors declare no competing financial interest. The funding sources had no involvement in the preparation of the article. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = jul,

doi = "10.1016/j.toxicon.2017.12.046",

language = "English",

volume = "149",

pages = "65--71",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier",

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T1 - ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa

T2 - A minireview

AU - Belyy, Alexander

AU - Mechold, Undine

AU - Renault, Louis

AU - Ladant, Daniel

N1 - Funding Information: The authors thank Jenny Lee Thomassin for critical reading of the manuscript. A.B. was supported by a stipend from the Pasteur-Paris University ( PPU ) International PhD Program. The authors were funded by CNRS and by the PTR program of the Institut Pasteur ( PTR425 and PTR43-16 ). The authors declare no competing financial interest. The funding sources had no involvement in the preparation of the article. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/7

Y1 - 2018/7

N2 - ExoY is one of four well-characterized Pseudomonas aeruginosa type 3 secretion system (T3SS) effectors. It is a nucleotidyl cyclase toxin that is inactive inside the bacteria, but becomes potently activated once it is delivered into the eukaryotic target cells. Recently, filamentous actin was identified as the eukaryotic cofactor that stimulates specifically ExoY enzymatic activity by several orders of magnitude. In this review, we discuss recent advances in understanding the biochemistry of nucleotidyl cyclase activity of ExoY and its regulation by interaction with filamentous actin.

AB - ExoY is one of four well-characterized Pseudomonas aeruginosa type 3 secretion system (T3SS) effectors. It is a nucleotidyl cyclase toxin that is inactive inside the bacteria, but becomes potently activated once it is delivered into the eukaryotic target cells. Recently, filamentous actin was identified as the eukaryotic cofactor that stimulates specifically ExoY enzymatic activity by several orders of magnitude. In this review, we discuss recent advances in understanding the biochemistry of nucleotidyl cyclase activity of ExoY and its regulation by interaction with filamentous actin.

KW - Actin

KW - Bacterial protein toxin

KW - ExoY

KW - Pseudomonas aeruginosa

KW - T3SS

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DO - 10.1016/j.toxicon.2017.12.046

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AN - SCOPUS:85039930034

SN - 0041-0101

VL - 149

SP - 65

EP - 71

JO - Toxicon

JF - Toxicon

ER -

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview

Abstract

Keywords

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