Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus

Michaela Wagner; Silvia Berkner; Malgorzata Ajon; Arnold J. M. Driessen; Georg Lipps; Sonja-Verena Albers

doi:10.1042/BST0370097

Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus

Michaela Wagner, Silvia Berkner, Malgorzata Ajon, Arnold J. M. Driessen, Georg Lipps, Sonja-Verena Albers^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. in the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.

Original language	English
Pages (from-to)	97-101
Number of pages	5
Journal	Biochemical Society Transactions
Volume	37
Issue number	1
DOIs	https://doi.org/10.1042/BST0370097
Publication status	Published - Feb-2009
Event	Conference on Molecular Biology of Archaea - Duration: 19-Aug-2008 → 21-Aug-2008

Keywords

archaeon
deletion mutant
genetics
plasmid
recombination
Sulfolobus
METHANOCOCCUS-VOLTAE
SHUTTLE VECTORS
ACIDOCALDARIUS
SOLFATARICUS
SYSTEM
RECOMBINATION
RESISTANCE
DNA

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Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus
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title = "Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus",

abstract = "Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. in the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.",

keywords = "archaeon, deletion mutant, genetics, plasmid, recombination, Sulfolobus, METHANOCOCCUS-VOLTAE, SHUTTLE VECTORS, ACIDOCALDARIUS, SOLFATARICUS, SYSTEM, RECOMBINATION, RESISTANCE, DNA",

author = "Michaela Wagner and Silvia Berkner and Malgorzata Ajon and Driessen, {Arnold J. M.} and Georg Lipps and Sonja-Verena Albers",

year = "2009",

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doi = "10.1042/BST0370097",

language = "English",

volume = "37",

pages = "97--101",

journal = "Biochemical Society Transactions",

issn = "0300-5127",

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note = "Conference on Molecular Biology of Archaea ; Conference date: 19-08-2008 Through 21-08-2008",

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T1 - Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus

AU - Wagner, Michaela

AU - Berkner, Silvia

AU - Ajon, Malgorzata

AU - Driessen, Arnold J. M.

AU - Lipps, Georg

AU - Albers, Sonja-Verena

PY - 2009/2

Y1 - 2009/2

N2 - Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. in the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.

AB - Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. in the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.

KW - archaeon

KW - deletion mutant

KW - genetics

KW - plasmid

KW - recombination

KW - Sulfolobus

KW - METHANOCOCCUS-VOLTAE

KW - SHUTTLE VECTORS

KW - ACIDOCALDARIUS

KW - SOLFATARICUS

KW - SYSTEM

KW - RECOMBINATION

KW - RESISTANCE

KW - DNA

U2 - 10.1042/BST0370097

DO - 10.1042/BST0370097

M3 - Article

VL - 37

SP - 97

EP - 101

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

IS - 1

T2 - Conference on Molecular Biology of Archaea

Y2 - 19 August 2008 through 21 August 2008

ER -